Protein Info for AO353_08195 in Pseudomonas fluorescens FW300-N2E3

Annotation: non-canonical purine NTP pyrophosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00042: non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family
  • amino acids 7 to 194 (188 residues), 185.7 bits, E=2.7e-59
• PF01725: Ham1p_like
  • amino acids 7 to 193 (187 residues), 211.8 bits, E=4.1e-67

Best Hits

Swiss-Prot: 89% identical to IXTPA_PSEPK: dITP/XTP pyrophosphatase (PP_5100) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01516, nucleoside-triphosphatase [EC: 3.6.1.15] (inferred from 92% identity to pfo:Pfl01_5326)

Predicted SEED Role

"Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15)" in subsystem Heat shock dnaK gene cluster extended (EC 3.6.1.15)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.15

Sequence Analysis Tools

See A0A0N9WAC2 at UniProt or InterPro

Protein Sequence (198 amino acids)

>AO353_08195 non-canonical purine NTP pyrophosphatase (Pseudomonas fluorescens FW300-N2E3)
MINFSQLVLASHNAGKLKELQAMLGESVHLRSIGEFSSVEPEETGLSFVENAILKARNAS
RISGLPALADDSGLAVDFLGGAPGIYSARYADGQGDAANNAKLLDVLKDVPEAERGAQFV
CVLALVRHADDPLPILCEGLWHGRILTEASGKHGFGYDPLFWVPERECSSAELSPAEKNQ
LSHRARAMDLLRQRLSLK