Protein Info for AO353_07970 in Pseudomonas fluorescens FW300-N2E3
Annotation: aromatic ring-opening dioxygenase LigA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 70% identical to LIGB_PSEPF: DNA ligase B (ligB) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 70% identity to pfo:Pfl01_5270)MetaCyc: 42% identical to DNA ligase B (Escherichia coli K-12 substr. MG1655)
DNA ligase (NAD(+)). [EC: 6.5.1.2, 6.5.1.6]
Predicted SEED Role
"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)
MetaCyc Pathways
- NAD salvage pathway I (PNC VI cycle) (5/7 steps found)
- NAD salvage pathway III (to nicotinamide riboside) (2/3 steps found)
- NAD salvage pathway II (PNC IV cycle) (3/5 steps found)
- NAD salvage (plants) (6/11 steps found)
Isozymes
Compare fitness of predicted isozymes for: 6.5.1.2
Use Curated BLAST to search for 6.5.1.2 or 6.5.1.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9W3U0 at UniProt or InterPro
Protein Sequence (560 amino acids)
>AO353_07970 aromatic ring-opening dioxygenase LigA (Pseudomonas fluorescens FW300-N2E3) MLPTLRLLIGFWLIVACLNANANSCPDWLPIRAQAEITALQAQIERWDDSYHRQGHSLVA DEIYDQSRAHLKAWRACFGGTTTPENPLHAARGTVTHPIPHTGLEKLIDGRAVEAWLKTH DDIWVQPKVDGVAVTLIYREGQFQQAISRGDGINGQDWTASARKIAAIPQQLPQSLDLLL QGELYWRLTDHVQAQRGSLNARSAVAGLMARKELSPVDANRIGLFVWDWPQGPASLPERL LALKALGFPDSEFYSQPVGAFADTERWRDHWYRSPLPFASDGIVLRQSQRPAAERWEAKT PFWSVAWKYPFAQALAEVRKVNFKIGRTGRITPVLELAPVTLDDRQIKRVSVSSLQRWQA MDIRPGDQVAISLAGLTIPRLDRVVLRSTERPEVNAPRAEDFHHLSCWQPTPGCESQFLA RLTWLSGKQGLALRHIGPGTWEKLISAGRLKGLLDWLTLDAQELANIDGFGERSSVRLLN SLQSARQQPFSRWLKALGLPPTGEASLADSWHLLVQRDTEQWQTEAGIGPGRAAQLSAFF RDPQVLALSEQLHAAGIDGF