Protein Info for AO353_07705 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): DgcB Dimethylglycine demethylase subunit B (EC 1.5.3.-)
Rationale: Specifically important for: Carnitine Hydrochloride. Dimethylglycine is an intermediate in L-carnitine metabolism; this gene is similar to PA5399 or dgcB (PMCID: PMC2293255)
Original annotation: (Fe-S)-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 649 transmembrane" amino acids 6 to 25 (20 residues), see Phobius details amino acids 68 to 87 (20 residues), see Phobius details amino acids 93 to 114 (22 residues), see Phobius details amino acids 135 to 153 (19 residues), see Phobius details amino acids 159 to 179 (21 residues), see Phobius details PF11982: DUF3483" amino acids 4 to 220 (217 residues), 290.5 bits, see alignment E=3.2e-90 PF13183: Fer4_8" amino acids 243 to 332 (90 residues), 37.4 bits, see alignment E=1e-12 PF13237: Fer4_10" amino acids 243 to 330 (88 residues), 39 bits, see alignment E=2.3e-13 PF13534: Fer4_17" amino acids 244 to 333 (90 residues), 28.3 bits, see alignment E=7e-10 PF13187: Fer4_9" amino acids 244 to 331 (88 residues), 30.6 bits, see alignment E=9.9e-11 PF02754: CCG" amino acids 399 to 477 (79 residues), 15.5 bits, see alignment E=5.3e-06 amino acids 521 to 607 (87 residues), 55.3 bits, see alignment E=2.1e-18

Best Hits

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a5225)

Predicted SEED Role

"Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF" in subsystem L-rhamnose utilization or Lactate utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZP8 at UniProt or InterPro

Protein Sequence (649 amino acids)

>AO353_07705 DgcB Dimethylglycine demethylase subunit B (EC 1.5.3.-) (Pseudomonas fluorescens FW300-N2E3)
MLNTLLPILLFAALGLAVLGALRRVAMWRRGRASKVDLIGGLLAMPKRYMVDLHHVVARD
KYIANTHVATAGGAVASIVLAILVHGFGLHNRFLGYALLLMTAVMFVGAIFVYLRRRNPP
ARLSKGPWMRLPKSLLAFSASFFLVTLPVAGILPENFGGWLLAAILGVGVLWGVSELFFG
MTWGGPMKHAFAGALHLAWHRRAERFGGGRSTGLKPLDLNDPQAPLGVEKPKDFTWNQLL
GFDACVQCGKCEAACPAFAAGQPLNPKKLIQDMVVGLAGGTDAKFAGSPYPGKAIGEHAG
NPHQPIVNGLVDAETLWSCTTCRACVEECPMMIEHVDAIVDMRRHLTLEKGATPNKGAEV
LENLIATDNPGGFAPGGRMNWAADLNLTLLSEKKSTDVLFWVGDGAFDMRNQRTLRAFVK
VLKAAKVDFAVLGLEERDSGDVARRLGDEATFQLLAKRNIQTLAKYSFNRIVTCDPHSFH
VLKNEYGAFDGNYLVQHHSTYMAEIIDAGALNLGQHKGDSVTYHDPCYLGRYNGEYEAPR
QVLRALGIEVKEMQRSGFRSRCCGGGGGAPITDIPGKQRIPDMRMDDIRETGAELVAVGC
PQCTAMLEGVVEPRPLIKDIAELVADALLEDAAPSKSPAPTKRQPAEAH