Protein Info for AO353_07670 in Pseudomonas fluorescens FW300-N2E3
Updated annotation (from data): L-threonine aldolase (EC 4.1.2.5)
Rationale: Specifically important for utilizing L-Threonine. Automated validation from mutant phenotype: the predicted function (4.1.2.5) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: threonine aldolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 87% identical to LTAE_PSEUN: Low specificity L-threonine aldolase (ltaE) from Pseudomonas sp. (strain NCIMB 10558)
KEGG orthology group: K01620, threonine aldolase [EC: 4.1.2.5] (inferred from 94% identity to pfl:PFL_5731)Predicted SEED Role
"Low-specificity L-threonine aldolase (EC 4.1.2.48)" (EC 4.1.2.48)
MetaCyc Pathways
- L-threonine degradation IV (2/2 steps found)
- glycine biosynthesis IV (1/1 steps found)
- superpathway of L-threonine metabolism (12/18 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.1.2.48, 4.1.2.5
Use Curated BLAST to search for 4.1.2.48 or 4.1.2.5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9VM68 at UniProt or InterPro
Protein Sequence (346 amino acids)
>AO353_07670 L-threonine aldolase (EC 4.1.2.5) (Pseudomonas fluorescens FW300-N2E3) MTDKSQQFASDNYSGICPEAWAAMEQANQGHQRAYGDDEWTARASDDFRKLFETDCEVFF AFNGTAANSLALSSLCQSYHSVICSETAHVETDECGAPEFFSNGSKLLIARTENGKLTPE SIREIALKRQDIHYPKPRVVTLTQATEVGSIYTPEEIRAISATCKELGLNLHMDGARFSN ACAFLNCSPADLTWKAGVDVLCFGGTKNGMAVGEAILFFNHKLAEDFDYRCKQAGQLASK MRFLSAPWVGLLENDAWLKHARHANYCAQLLAELVSDIPGVELMFPVQANGVFLQLSESA VAALTAKSWRFYTFIGKGGARFMCSWDTEVERVRELAADIREVMAG