Protein Info for AO353_07485 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): Acyl-CoA dehydrogenase (EC 1.3.8.7)
Rationale: Specifically important for: Sodium butyrate; Tween 20. The phenotype on Tween 20 suggests that it has activity on longer substrates as well as on butyryl-CoA (SEED_correct)
Original annotation: acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 592 PF12418: AcylCoA_DH_N" amino acids 3 to 33 (31 residues), 44.9 bits, see alignment (E = 2.4e-15) PF02771: Acyl-CoA_dh_N" amino acids 38 to 158 (121 residues), 43.7 bits, see alignment E=8.4e-15 PF02770: Acyl-CoA_dh_M" amino acids 163 to 273 (111 residues), 48.5 bits, see alignment E=2e-16 PF00441: Acyl-CoA_dh_1" amino acids 283 to 451 (169 residues), 73 bits, see alignment E=8e-24 PF12806: Acyl-CoA_dh_C" amino acids 468 to 586 (119 residues), 120.6 bits, see alignment E=1.1e-38

Best Hits

KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 97% identity to pfo:Pfl01_5166)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.-

Use Curated BLAST to search for 1.3.8.7 or 1.3.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WH75 at UniProt or InterPro

Protein Sequence (592 amino acids)

>AO353_07485 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Pseudomonas fluorescens FW300-N2E3)
MADYKAPLRDMRFVLNEVFEVAKLWAELPALADTVDAETVEAILEEAGKVTSKSIAPLSR
AADEEGCHWSDGAVTTPAGFPQAYQTYAEGGWVGVGGDPSYGGMGMPKAVSAQVEEMVNS
ASLSFGLYPMLTAGACLSINAHASEELKAAYLPNMYAGVWAGSMCLTEPHAGTDLGIIRT
KAEPQADGSYKVSGTKIFITGGEHDLTENIIHLVLAKLPDAPAGPKGISLFLVPKFMVNA
DGSLGARNPANCGSIEHKMGIQASATCVMNFDEAVGYLVGEPNKGLAAMFTMMNYERLGV
GIQGLASGERSYQNAVEYARDRLQSRSPTGAQNKDKVADPIIVHPDVRRMLLTMKASNEG
GRAFSTYVAMQLDTAKFSEDATIRKRAEDLVALLTPVAKAFLTDLGLETTIHGQQVFGGH
GYIREWGQEQLVRDVRITQIYEGTNGIQALDLVGRKIVGSGGAYYKLFADEIRHFTATAS
ADLAEFTKPLNDAVDTLDELTAWLLDRVKNNPNEIGAASVEYLQVFGYVSYAYMWALMAK
AAFGKEAQDDFYASKLGTARFYFARLLPRFHSLSASVKAGSESLFLLDAAQF