Protein Info for AO353_07420 in Pseudomonas fluorescens FW300-N2E3
Updated annotation (from data): Lysine 2-monooxygenase (EC 1.13.12.2)
Rationale: Specifically important for utilizing L-Lysine. Automated validation from mutant phenotype: the predicted function (LYSINE-2-MONOOXYGENASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: amine oxidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00466, tryptophan 2-monooxygenase [EC: 1.13.12.3] (inferred from 97% identity to pfl:PFL_5670)MetaCyc: 90% identical to L-lysine monooxygenase monomer (Pseudomonas putida)
Lysine 2-monooxygenase. [EC: 1.13.12.2]
Predicted SEED Role
"Lysine 2-monooxygenase (EC 1.13.12.2)" in subsystem Lysine degradation (EC 1.13.12.2)
MetaCyc Pathways
- L-lysine degradation IV (5/5 steps found)
- indole-3-acetate biosynthesis III (bacteria) (2/2 steps found)
- superpathway of L-lysine degradation (18/43 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.13.12.3
Use Curated BLAST to search for 1.13.12.2 or 1.13.12.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N7GZP1 at UniProt or InterPro
Protein Sequence (560 amino acids)
>AO353_07420 Lysine 2-monooxygenase (EC 1.13.12.2) (Pseudomonas fluorescens FW300-N2E3) MNKNNRHPADGKKPVTIFGPDFPFAFDDWIEHPAGLGSIPAHNHGAEVAIVGAGIAGLVA AYELMKLGLKPVVYEASKMGGRLRSQAFEGAEGIIAELGGMRFPVSSTAFYHYVDKLGLE TKPFPNPLTPASGSTVIDLEGQTHYAQKLADLPALFQEVADAWADALEDGSRFGEIQQAI RDRDVPRLKELWNTLVPLWDDRTFYDFVATSKAFAKLSFHHREVFGQVGFGTGGWDSDFP NSMLEIFRVVMTNCDDHQHLVVGGVEQVPLGIWRHVPKRCAHWPEGTSLSSLHNGAPRTG VKRIARAADGRFSVTDNWGDTREYAAVLTTCQSWLLTTQIECEESLFSQKMWMALDRTRY MQSSKTFVMVDRPFWKDKDPETGRDLMSMTLTDRLTRGTYLFDNGDDKPGVICLSYSWMS DALKMLPQPIEKRVKLALDALKKIYPKVDIAARIIGDPITVSWEADPHFLGAFKGALPGH YRYNQRMYAHFMQQDMPAEQRGIFIAGDDVSWTPAWVEGAVQTSLNAVWGIMNHFGGKTH VENPGPGDVFHEIGPIALPE