Protein Info for AO353_07295 in Pseudomonas fluorescens FW300-N2E3

Annotation: aminoglycoside phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 341 PF01636: APH" amino acids 36 to 258 (223 residues), 147.7 bits, see alignment E=2.6e-47

Best Hits

Swiss-Prot: 76% identical to AMGK_PSEPK: N-acetylmuramate/N-acetylglucosamine kinase (amgK) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K07102, (no description) (inferred from 92% identity to pfo:Pfl01_5131)

MetaCyc: 76% identical to N-acetyl-D-muramate 1-kinase (Pseudomonas putida KT2440)
RXN-18660 [EC: 2.7.1.221]; N-acetylhexosamine 1-kinase. [EC: 2.7.1.221, 2.7.1.162]; N-acetylgalactosamine kinase. [EC: 2.7.1.221, 2.7.1.162, 2.7.1.157]

Predicted SEED Role

"COG3178: Predicted phosphotransferase related to Ser/Thr protein kinases" in subsystem YjeE

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.157 or 2.7.1.162 or 2.7.1.221

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WH39 at UniProt or InterPro

Protein Sequence (341 amino acids)

>AO353_07295 aminoglycoside phosphotransferase (Pseudomonas fluorescens FW300-N2E3)
MPDQDVRLQHLKVWLDEQLATLFATQGWGAVPSATLTAASSDASFRRYFRWEGDGRSFVV
MDAPPPQENCKPFVDIAFLLAKSGINVPKIYAQDLERGFLLLNDLGNKTYLDVIDNENAD
DLFKDALQALLAFQQLPMDAPLPSYDVALLRRELELFPEWYVKRELGIEFDPAQQMLWQQ
VSDKLIDSALAQPKVLVHRDYMPRNLMLSEPNPGVLDFQDAVYGPVTYDVTCLFKDAFLS
WPEERVRAWLANYWQQAGALGIPVQPDFEDFLRASDLMGVQRHLKVIGIFARICHRDGKP
RYLGDVPRFFAYIEAVIARRPELVELDVLLASLRQSVGVTA