Protein Info for AO353_07225 in Pseudomonas fluorescens FW300-N2E3

Annotation: esterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00657: Lipase_GDSL" amino acids 33 to 315 (283 residues), 54.5 bits, see alignment E=2.8e-18 PF03797: Autotransporter" amino acids 365 to 616 (252 residues), 161.7 bits, see alignment E=4.4e-51

Best Hits

Swiss-Prot: 67% identical to ESTA_PSEAE: Esterase EstA (estA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K12686, outer membrane lipase/esterase (inferred from 83% identity to pfo:Pfl01_5117)

Predicted SEED Role

"Phospholipase/lecithinase/hemolysin"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VSG3 at UniProt or InterPro

Protein Sequence (636 amino acids)

>AO353_07225 esterase (Pseudomonas fluorescens FW300-N2E3)
MIKQTMFVPLASCVLAMACAQAIAAPAPYTKFIVFGDSLNDAGQFTDTGGPAGATERFTN
RVGPTYKDGNGEVRATNSTQLLGGKLGFSPDQTAASTSAVRASEGLPDGNNWAVGGYRTD
QILDSITTISDTGERSRPGYLPSNNFRADPNALYYLSGGGNDFLQGRVTSLDQANAAADR
LVSSVQTLQQAGAKYIMVWLLPDIGLTPAINGSPLQAFTSQLSAQFNTELVSQLQHVNAE
VIPLNIPVLLKETFANPAQFGLATDQNLTATCFSGDSCTENARYGINSATPDPTKLIYND
SVHPTETGQRLIADYAYSILAAPWEMTLLPEMAHATLRAHQDELRNQWQSDWENWQNVGQ
WRAIVAGGGQHLNFDDQRSAASADGNGYNLNIGGSYRLDEAWRVGVAAGFYRQKLEAGND
NSQYKLNTYMGTAFAQYQQNRWWADAAMTAGHLDYDSLKRKFDLGVNERGEKGSTNGTVL
AVTGRLGYDIAQQASSPWHLSPFISADYAKVQVDGYSENGNDSTALTFGDQKRTSKRLGV
GLQGKYQITSQTQVFGEFAHEREYKDDAQDLTMNLNSLPDNHFTLQGYTPQTNLNRLNLG
VSQKLTPDLALRASYNIRKDDDFTQQGINVGVSLDF