Protein Info for AO353_07135 in Pseudomonas fluorescens FW300-N2E3

Annotation: tyrosine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 399 TIGR00234: tyrosine--tRNA ligase" amino acids 2 to 396 (395 residues), 402.3 bits, see alignment E=1.3e-124 PF00579: tRNA-synt_1b" amino acids 31 to 310 (280 residues), 290.5 bits, see alignment E=7.4e-91

Best Hits

Swiss-Prot: 96% identical to SYY_PSEPF: Tyrosine--tRNA ligase (tyrS) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 95% identity to pfl:PFL_5607)

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WDC1 at UniProt or InterPro

Protein Sequence (399 amino acids)

>AO353_07135 tyrosine--tRNA ligase (Pseudomonas fluorescens FW300-N2E3)
MKSVEEQLALIKRGAEELLVESELIEKLKRGQPLRIKAGFDPTAPDLHLGHTVLINKLRQ
FQDLGHQVIFLIGDFTGMIGDPSGKSATRPPLTREQVLENAETYKTQVFKILDPAKTEVA
FNSTWMDQMGPADFIRLTSQYTVARMLERDDFDKRYSTNQPIAIHEFLYPLVQGYDSVAL
RADVELGGTDQKFNLLMGRELQRSYGQEAQCILTMPLLEGLDGVKKMSKSLGNYVGIQEA
PGVMYGKLVSIPDVLMWRYFELLSFRSMEEIDALRADVEAGANPRDIKIKLAEEIVARFH
GEEAAANAHRGAGNRMKEGELPDDLPEIELTATEDMPIAAVLNKAGLVKNSAVARDLLGS
GGVRIDGEVVDRTFIYALGATHVCQAGKKAFARITLKSE