Protein Info for AO353_06780 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): DNA repair helicase Lhr
Rationale: Specifically important in stress Cisplatin. Similar to the full length of Lhr from Mycobacterium smegmatis, which is a helicase that is suspected to be involved in DNA repair (see PMC3656269) (SEED_correct)
Original annotation: ATP-dependent DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1438 PF00270: DEAD" amino acids 30 to 216 (187 residues), 96.2 bits, see alignment E=5.5e-31 PF04851: ResIII" amino acids 34 to 215 (182 residues), 33.4 bits, see alignment E=1.3e-11 PF00271: Helicase_C" amino acids 273 to 371 (99 residues), 63.5 bits, see alignment 6.1e-21 PF08494: DEAD_assoc" amino acids 636 to 838 (203 residues), 165 bits, see alignment E=5.6e-52

Best Hits

Predicted SEED Role

"Lhr-like helicases"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WGU7 at UniProt or InterPro

Protein Sequence (1438 amino acids)

>AO353_06780 DNA repair helicase Lhr (Pseudomonas fluorescens FW300-N2E3)
MNLPVSAESALAGFHPAVRAWFNSTFPAVTAAQAGAWPLIRQRRSTLVAAPTGSGKTLTA
FLAVIDDLVHQGLAHGGELPDETLVVYVSPLKALSNDIQINLQTPLAGITEQLRELGFPE
LQINTAVRTGDTPQKDRSAMRKSAPHILVTTPESLYVLLGSDSGRRMLASTRTVIVDEIH
AIAGSKRGSHLALSLERLQALCCEPLMRIGLSATQKPIEAVSRFLVGTGRPCEIIDIGHA
RRRDLGIEVPPVPLSAVMANDVWELVYDRIAELAREHRTTLIFVNTRRLAERLSRHLSER
LGKDAVAAHHGSLAKEFRLDAEQRLKRGDLQVLIATASLELGIDIGDVDLVCQIASPRSI
AGFLQRVGRSGHQVGGTPKGRLFATTRDDLIECAALLDCVRRGELDTLLIPHAPLDVLAQ
QIVAEVSCQEWQERALLEMVRRASPYTELDDTHYQALLQMLAEGYNGRQGIRSAYLHRDA
VTRTLRGRRGAKLTAVTSGGTIPDNADYSVLLEPQGLNIGSVNEDFAVESIAGDVFQLGN
TSYRILRVETGKIRVEDAQGQPPTIPFWLGEAPGRSAELSLAVARLHAHLDELLGATPGD
VQPSIDWLTETLGLNLASAEQLVDYLARARLTLGALPSQNTLLMERFFDESGGTQLIIHS
LFGSRINRAWGLALRKRFCRTFNFELQAAASEDAIVLSLSTSHSFELDEVWRYLHSNTAE
QILIQAVLDAPLFGVRWRWNAGVALALPRYSGGRKVPPQIQRMKSEDLIASVFPDQIACL
ENLVGEREIPDHPLVEQTLDDCLHEAMDCEGWLALLRRMESGEVRLISRDLPAPSPLAAE
ILSARPYTFLDDAPLEERRTQAVLNRRWSDPQSTDDLGALDAEAIQSVRDEAWPQPTSVD
EMHEALMSLAAISNAEALANPQWSHWLQTLADSGRASRLQINAEHSLWLALERLTCLQAI
YPHATLLPPLQALPGFDEPWDVDEALVELIRARLSAFGPLPIHAIAQPLGLSTAQVTQAL
AQLEREGYVLRGRFSPAAPQEEWCERHLLARIHRYTVKRLRREIEPVALQDFMRFLFDWQ
HLSPATQGQGSAVLPAIVSQFEGYPAAASAWDSDLLPARLKNYSSTWLDELCRSGKLVWT
RLTVRNKATSAALRSTPIVLLPRAQVPLWSSLTEPPEVSELSPKTQKVHQALSQHGALFF
DELLHEAHLLRSELEIALQELVGAGLVNADSFAGLRALITPASKRQARSSRRGRGAFVGG
MDDAGRWALLRRQSTPPVVDNRGTPSETLEHIALTLLRRYGVVFWRLLEREAEWLPSWRE
LLRTFHRLEARGEIRGGRFVSGLAGEQFALPEAIPLLREVRRRQHDGSLISVCGVDPLNL
AGTLLPGHKVPALASNRLVYRDGLPAAAEIAGTQHYWLELDQPNTAELHKKLTQYRTL