Protein Info for AO353_06775 in Pseudomonas fluorescens FW300-N2E3

Annotation: dTDP-4-dehydrorhamnose reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 3 to 290 (288 residues), 267.2 bits, E=1.5e-83
• PF01370: Epimerase
  • amino acids 40 to 118 (79 residues), 21.9 bits, E=1e-08

Best Hits

KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 95% identity to pfo:Pfl01_5031)

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (5/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.133

Use Curated BLAST to search for 1.1.1.133

Sequence Analysis Tools

See A0A0N9VS99 at UniProt or InterPro

Protein Sequence (294 amino acids)

>AO353_06775 dTDP-4-dehydrorhamnose reductase (Pseudomonas fluorescens FW300-N2E3)
MRMRLMLLGGGNALGQALIRLGAEEDIGFLAPRPPQDGWDAASLTQLLDDTRPDALINLA
YYFDWFQAESVAEARLAAQERAVERLAELCQHHNIVLVQPSSYRVFDGSRATAYSEKDEP
VPLGLRGQALWRIEQSVRATCPQHVMLRFGWLLDDSVDGTLGRFLARAELPEELLMADDR
RGNPTPVDDAARVIISVLKQLDCAAPLWGTYHYAGHEATTPLALGQAILAEARTLHPLAI
EAPTAQAHAARPDAAEEPQHAVLACKKILHTFGIKPRAWRAALPGLLDRFYRHG