Protein Info for AO353_06515 in Pseudomonas fluorescens FW300-N2E3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 238 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 42 to 66 (25 residues), see Phobius details amino acids 72 to 91 (20 residues), see Phobius details amino acids 99 to 124 (26 residues), see Phobius details amino acids 151 to 177 (27 residues), see Phobius details amino acids 189 to 206 (18 residues), see Phobius details amino acids 212 to 235 (24 residues), see Phobius details PF04172: LrgB" amino acids 23 to 235 (213 residues), 251.5 bits, see alignment E=2.8e-79

Best Hits

Swiss-Prot: 42% identical to YOHK_SHIFL: Inner membrane protein YohK (yohK) from Shigella flexneri

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_4980)

Predicted SEED Role

"LrgA-associated membrane protein LrgB" in subsystem Murein hydrolase regulation and cell death

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VRB4 at UniProt or InterPro

Protein Sequence (238 amino acids)

>AO353_06515 hypothetical protein (Pseudomonas fluorescens FW300-N2E3)
MIFDWQGALAAVIHHPLFGIGVTLGAYQLVLAAFEKTRWIFLQPVLVSMLLVIGVLLACG
LTYAEYRKSTEILSILLGPATVALAVPLYLNLRRIRQLFWPIFTTLVIGGVVATGMGVLL
GWWFGAEHMILMTMAPKSVTSPIAMLVAEQIGGVAALAAVFVLITGVIGAIFGPSLLNRL
GVHSPEARGMALGMTAHAVGTAVAMQESEECGAFAALAMSLMGVATAVFLPLAVSMVV