Protein Info for AO353_06470 in Pseudomonas fluorescens FW300-N2E3

Annotation: rod shape-determining protein RodA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 43 to 63 (21 residues), see Phobius details amino acids 71 to 93 (23 residues), see Phobius details amino acids 108 to 126 (19 residues), see Phobius details amino acids 135 to 153 (19 residues), see Phobius details amino acids 158 to 176 (19 residues), see Phobius details amino acids 182 to 200 (19 residues), see Phobius details amino acids 270 to 291 (22 residues), see Phobius details amino acids 303 to 328 (26 residues), see Phobius details amino acids 336 to 357 (22 residues), see Phobius details TIGR02210: rod shape-determining protein RodA" amino acids 14 to 362 (349 residues), 439.5 bits, see alignment E=4.9e-136 PF01098: FTSW_RODA_SPOVE" amino acids 21 to 362 (342 residues), 370.3 bits, see alignment E=4.8e-115

Best Hits

Swiss-Prot: 53% identical to RODA_ECOLI: Peptidoglycan glycosyltransferase MrdB (mrdB) from Escherichia coli (strain K12)

KEGG orthology group: K05837, rod shape determining protein RodA (inferred from 96% identity to pfl:PFL_5451)

MetaCyc: 53% identical to peptidoglycan glycosyltransferase MrdB (Escherichia coli K-12 substr. MG1655)
Peptidoglycan glycosyltransferase. [EC: 2.4.1.129]

Predicted SEED Role

"Rod shape-determining protein RodA" in subsystem Bacterial Cytoskeleton or Peptidoglycan Biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129

Use Curated BLAST to search for 2.4.1.129

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WS75 at UniProt or InterPro

Protein Sequence (367 amino acids)

>AO353_06470 rod shape-determining protein RodA (Pseudomonas fluorescens FW300-N2E3)
MRRRATLLQRLHIDGPLLILLLILAAGSLFVLYSASGKSWDLLAKQATSFGIGLVSMIVI
AQFEPRFMARWVPLGYVLGVILLVVVDVMGHNAMGATRWINIPGVIRFQPSEFMKILMPA
TIAWYLSKRTLPPQLKHVGVSLFLIGLPFILIVRQPDLGTSLLILAGGAFVLFMGGLRWR
WILSVVAAAVPVAVAMWFFIMHDYQKQRILTFLDPESDPLGTGWNIIQSKAAIGSGGVFG
KGWLLGTQSHLDFLPESHTDFIIAVMGEEFGLVGICALLLIYLLLIGRGLVITAQAQTLF
GKLLAGSLTMTFFVYVFVNIGMVSGLLPVVGVPLPFISYGGTSLVTLLSAFGVLMSIHTH
RKWIAQV