Protein Info for AO353_06460 in Pseudomonas fluorescens FW300-N2E3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 336 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF03330: DPBB_1" amino acids 100 to 189 (90 residues), 91.8 bits, see alignment E=2.6e-30 TIGR00413: rare lipoprotein A" amino acids 102 to 193 (92 residues), 145.7 bits, see alignment E=9e-47 PF05036: SPOR" amino acids 262 to 329 (68 residues), 46 bits, see alignment E=5.6e-16

Best Hits

Swiss-Prot: 68% identical to RLPA_PSEAB: Endolytic peptidoglycan transglycosylase RlpA (rlpA) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K03642, rare lipoprotein A (inferred from 95% identity to pfo:Pfl01_4967)

Predicted SEED Role

"Rare lipoprotein A precursor" in subsystem Peptidoglycan Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WFI9 at UniProt or InterPro

Protein Sequence (336 amino acids)

>AO353_06460 hypothetical protein (Pseudomonas fluorescens FW300-N2E3)
MLASPIRKPLKLVAFAALSLLVVSCSTSRAPQQKYTTAVRATPGLDINRAHKDGAPWWDV
DISRIPDATPTLHTGPYKANPYTVLGKTYFPLAESKRYVASGTASWYGTKFHGQNTANGE
VYDLYGMSAAHKTLPLPSYVRVTNLDNNKSVILRVNDRGPFYSDRIIDLSYAAAKKLGYA
EIGTARVKVEGIDPQEWWAAKGRPAPLMLNEPQVAQNSAPVITASAGTVEQWTPPPQQHA
AAVVPVQLDAKKNASVPASGQYLQVGAFANPDAAELLRSKLSGMVSAPVFISSIVRNQQT
LHRVRLGPIGSQGEIAQVQNSVRLANLGSPSLVTAE