Protein Info for AO353_06360 in Pseudomonas fluorescens FW300-N2E3

Annotation: glutamate-1-semialdehyde 2,1-aminomutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00713: glutamate-1-semialdehyde-2,1-aminomutase
  • amino acids 4 to 424 (421 residues), 633.5 bits, E=7.1e-195
• PF00202: Aminotran_3
  • amino acids 32 to 394 (363 residues), 261.8 bits, E=9.6e-82
• PF00155: Aminotran_1_2
  • amino acids 182 to 399 (218 residues), 22.2 bits, E=7.6e-09

Best Hits

Swiss-Prot: 97% identical to GSA_PSEF5: Glutamate-1-semialdehyde 2,1-aminomutase (hemL) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01845, glutamate-1-semialdehyde 2,1-aminomutase [EC: 5.4.3.8] (inferred from 97% identity to pfl:PFL_5429)

MetaCyc: 66% identical to glutamate-1-semialdehyde 2,1-aminomutase subunit (Salmonella enterica enterica serovar Typhimurium)
Glutamate-1-semialdehyde 2,1-aminomutase. [EC: 5.4.3.8]

Predicted SEED Role

"Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 5.4.3.8)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• superpathway of heme b biosynthesis from glutamate (9/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.3.8

Sequence Analysis Tools

See A0A0N9WGJ8 at UniProt or InterPro

Protein Sequence (427 amino acids)

>AO353_06360 glutamate-1-semialdehyde 2,1-aminomutase (Pseudomonas fluorescens FW300-N2E3)
MSRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAEGAYVTDEDDKRYVDYVGSWG
PMILGHSHPDVLDAVRKQLEHGLSYGAPTAMETEMADLVCSIVPSMEMVRMVSSGTEATM
SAIRLARGFTGRDSIIKFEGCYHGHSDSLLVKAGSGLLTHGVPSSAGVPAAFAKHTLTLP
FNDIDAVEKMLAEVGQEVACIIVEPVAGNMNCVPPAPGFLEGLRTLCDKHGVVLIFDEVM
TGFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKREIMSHIAPLGPVYQAGTLSG
NPLAMAAGLTTLRLISRPGFHAELSEYTSRLLDGLQQRADAAGIPFVTTQAGGMFGLYFS
GADDIVTFDDVMASDANLFKRFFHLMLEGGVYLAPSAFEAGFTSIAHGDAELKLTLDAAE
RAFAALK