Protein Info for AO353_06300 in Pseudomonas fluorescens FW300-N2E3

Annotation: ATP-dependent helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 843 TIGR01970: ATP-dependent helicase HrpB" amino acids 4 to 842 (839 residues), 1247 bits, see alignment E=0 PF00270: DEAD" amino acids 16 to 163 (148 residues), 38.5 bits, see alignment E=2.1e-13 PF00271: Helicase_C" amino acids 208 to 333 (126 residues), 48.2 bits, see alignment E=2.4e-16 PF08482: HrpB_C" amino acids 699 to 830 (132 residues), 180 bits, see alignment E=5.4e-57

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 92% identity to pba:PSEBR_a4929)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VS00 at UniProt or InterPro

Protein Sequence (843 amino acids)

>AO353_06300 ATP-dependent helicase (Pseudomonas fluorescens FW300-N2E3)
MNSLPIDEVLPALREALATRHEAVLEAPPGAGKTTRVPLAMLDEPWLAGQTILMLEPRRL
AARAAAERLASELGEKVGETVGYRIRLDSKVGPNTRIEVVTEGILTRRLQEDPALDGVGL
LIFDEYHERSLDADLALALSLNGRELFRDEQPLKILLMSATLEGERLAGLLNDAPILRSE
GRMYPVTMRWGRPFQPGEFIEPRVVQTILDALNDEAGSLLVFLPGQAEIRRVHQQLADAL
GERSEVLLCPLHGELDLAAQRAAIDPAPPGQRKVVLATNIAETSLTINGVRVVIDAGLAR
VPRFDPGSGMTRLDTQRISRASATQRAGRAGRLEPGVCYRLWSQDQHEQLAAYASPEILS
ADLAGLALQLGRWGVTPGQLIWLDVPPAAAYAQAQDLLERLGALQGSSAEDWKLTRHGQA
MAELPAHPRIAHLLLRGQALGLASMACDVAALLGERDILRGVGADLHSRLVLLSGEERAA
RGAQGGVQRARQLARQYRGYLRGKAESPVSDPEHPRWLGALLALAYPDRVAQQRRAGGAE
YRLANGRAALFAEADSLMKQPWLVIADLGSRQGQREERIYLAADFDPALFDSVLAEQVRC
VDQLDWDEREGVLRAERQRKVGELVLSREPLTGLDETARSQALVNLVRRKGLELLPWTPE
LRQWQARVALLRQLDLNKQGESEWPDVSDAALLASLEHWLMPYLGRVSRLSHFASLDVSS
FVHNLLPWPLPQRLDEQAPHHLSVPSGSSIRLDYSEQPPILAVRLQELFGLAETPRIAGG
RQVVKLHLLSPARRPVQVTQDLANFWRSTYAEVKKDLKGRYPKHYWPDDPLVAEATARAK
PRK