Protein Info for AO353_06275 in Pseudomonas fluorescens FW300-N2E3

Annotation: DNA polymerase III subunit epsilon

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 203 PF00929: RNase_T" amino acids 6 to 160 (155 residues), 96.7 bits, see alignment E=1.1e-31

Best Hits

KEGG orthology group: K02342, DNA polymerase III subunit epsilon [EC: 2.7.7.7] (inferred from 95% identity to pba:PSEBR_a4924)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VR50 at UniProt or InterPro

Protein Sequence (203 amino acids)

>AO353_06275 DNA polymerase III subunit epsilon (Pseudomonas fluorescens FW300-N2E3)
LERIAVIDFETTGISPSSSCRATEIAVVILEQGRIVDRYQSLMNAGVRIPAFIEQLTGIS
NAMLRTAPSAEQVMNEVNEFVGITPLLAHNAAFDQKFWDFELGRIKRTRLQNFACSLLLA
RRLMPAAPNHKLGTLTTFAQLPHTGQAHRAMADAEMAANLTAHLAQELRQKHGLRELSHD
LLCSLQKVPAAKINEHLKHHRGF