Protein Info for AO353_06040 in Pseudomonas fluorescens FW300-N2E3

Annotation: exodeoxyribonuclease V subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 695 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR01447: exodeoxyribonuclease V, alpha subunit" amino acids 34 to 694 (661 residues), 649.2 bits, see alignment E=3.2e-199 PF21185: RecD_N" amino acids 36 to 157 (122 residues), 43 bits, see alignment E=2.1e-14 PF13245: AAA_19" amino acids 206 to 358 (153 residues), 111.4 bits, see alignment E=1.7e-35 PF13604: AAA_30" amino acids 209 to 372 (164 residues), 96.5 bits, see alignment E=8.1e-31 PF13086: AAA_11" amino acids 211 to 292 (82 residues), 29.6 bits, see alignment E=2.8e-10 PF13401: AAA_22" amino acids 217 to 350 (134 residues), 33 bits, see alignment E=3.2e-11 PF05127: Helicase_RecD" amino acids 220 to 340 (121 residues), 40 bits, see alignment E=1.8e-13 PF13538: UvrD_C_2" amino acids 622 to 668 (47 residues), 44 bits, see alignment 7.1e-15

Best Hits

KEGG orthology group: K03581, exodeoxyribonuclease V alpha subunit [EC: 3.1.11.5] (inferred from 84% identity to pba:PSEBR_a684)

Predicted SEED Role

"Exodeoxyribonuclease V alpha chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VRV9 at UniProt or InterPro

Protein Sequence (695 amino acids)

>AO353_06040 exodeoxyribonuclease V subunit alpha (Pseudomonas fluorescens FW300-N2E3)
MSRSFADLLPTPLTAESLAQLTPLSRADDLLLLLERWVERGWLRALDKAFVAFLHELAPD
GDPLVLLAAALTSHQLGHGHVCLDLFETLKEPDFALSLPPEGDLQTGTLLLPSQLLEALE
GAHWCKVLASSSLVALAVDERDESQQRPLVLSGKRLYLRRYWTYERRIDSALRQRLIVHE
PTPDDLSERLSGLFGQASSSAQIDWQKLACALATRGAFSIVTGGPGTGKTTTVVRLLALL
QAPAVEAGKPLRIRLAAPTGKAAARLTESISQQVRTLQVAESVRDKIPSDVTTVHRLLGS
RPGTRHFRHHAGNRLPLDVLVVDEASMIDLEMMANLLDALPTHARLVLLGDKDQLASVEA
GAVLGDLCRDAEAGWYSPQTRDWLEAVSGENLGSSGLQEDIDATHPLAQQVVMLRHSRRF
GEGSGIGQLARWVNQQQADEARKLLAARSHADLFSLPLKGEQDRALERLLLEGHGEGPQG
YRHYLSLLRNQRPSINSLPDDPCWIEWARNVLQAFDAFQLLCAVRKGPWGVEGLNQRITG
ALLKARLIDSDQQWYEGRPVLMTRNDYGLGLMNGDIGIALKLPERDGQEGGRQVLRVAFA
RNDGQGGVRFVLPSRLNDVETVYAMTVHKSQGSEFAHTALILPDALNPVLTKELIYTGIT
RAKNWFSLIEPRTGIFEEAVRRKVKRLSGLMLELG