Protein Info for AO353_05990 in Pseudomonas fluorescens FW300-N2E3
Updated annotation (from data): Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)
Rationale: Specifically important for utilizing L-Valine. Automated validation from mutant phenotype: the predicted function (RXN-11213) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: methylmalonate-semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to MMSA_ARATH: Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (ALDH6B2) from Arabidopsis thaliana
KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 97% identity to pfo:Pfl01_0695)MetaCyc: 72% identical to methylmalonate-semialdehyde dehydrogenase subunit (Burkholderia cepacia R34)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]
Predicted SEED Role
"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)
MetaCyc Pathways
- myo-inositol degradation I (7/7 steps found)
- β-alanine degradation II (2/2 steps found)
- L-valine degradation I (6/8 steps found)
- myo-, chiro- and scyllo-inositol degradation (7/10 steps found)
- β-alanine degradation I (1/2 steps found)
- propanoyl-CoA degradation II (3/5 steps found)
- 2,4-dinitrotoluene degradation (1/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.27
Use Curated BLAST to search for 1.2.1.27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9WGB5 at UniProt or InterPro
Protein Sequence (507 amino acids)
>AO353_05990 Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (Pseudomonas fluorescens FW300-N2E3) MNASLTPETTVQKVKLLIDGQWVESQTTEWHDIVNPATQQVLAKVPFATPAEVDAAISAA HRAFQTWKLTPIGARMRIMLKLQALIREHSKRIAVVLSAEQGKTIADAEGDIFRGLEVVE HACSIGSLQMGEFAENVAGGVDTYTLRQPIGVCAGITPFNFPAMIPLWMFPMAIACGNTF VLKPSEQDPMSTMLLVELAIEAGIPAGVLNVVHGGKDVVDALCTHKDIKAVSFVGSTAVG THVYDLAGKHGKRVQSMMGAKNHAVVLPDANREQALNALVGAGFGAAGQRCMATSVVVLV GAAKQWLPDLKALAQKLKVNAGSEPGTDVGPVISKRAKARILDLIESGIKEGAKLELDGR DITVPGYEKGNFVGPTLFSGVTTDMQIYTQEIFGPVLVVLEVDTLDQAIALVNANPFGNG TGLFTQSGAAARKFQNEIDVGQVGINIPIPVPVPFFSFTGSRGSKLGDLGPYGKQVVQFY TQTKTVTSRWFDDDSVNDGVNTTINLR