Protein Info for AO353_05775 in Pseudomonas fluorescens FW300-N2E3

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details amino acids 53 to 75 (23 residues), see Phobius details amino acids 87 to 107 (21 residues), see Phobius details amino acids 113 to 135 (23 residues), see Phobius details amino acids 153 to 175 (23 residues), see Phobius details amino acids 187 to 206 (20 residues), see Phobius details amino acids 239 to 264 (26 residues), see Phobius details amino acids 277 to 297 (21 residues), see Phobius details amino acids 305 to 322 (18 residues), see Phobius details amino acids 334 to 355 (22 residues), see Phobius details amino acids 367 to 389 (23 residues), see Phobius details amino acids 401 to 418 (18 residues), see Phobius details PF00083: Sugar_tr" amino acids 17 to 416 (400 residues), 98.4 bits, see alignment E=4.5e-32 PF07690: MFS_1" amino acids 23 to 371 (349 residues), 109.9 bits, see alignment E=1.3e-35

Best Hits

KEGG orthology group: K03762, MFS transporter, MHS family, proline/betaine transporter (inferred from 88% identity to pen:PSEEN2545)

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VQW7 at UniProt or InterPro

Protein Sequence (446 amino acids)

>AO353_05775 MFS transporter (Pseudomonas fluorescens FW300-N2E3)
VGIQQKNPARQRRRAFIGATSGHLIEWYDYGVYGFLAVYIGKAFFVSDDPTSSLLASFAA
FALSFFIRPLGGLFFGPLADKIGRRKTLITVLVMMAGSTVLLGLLPTYATLGIAAPILLV
LIRCVQGFSAGGEIGTITSFISEYAGPGRRGFATCWLMVTAVLGLLLGGAVANGMTWVMG
ADLMQAWGWRIPFLIAAPLGLISMYIRLKLEDSPEFLALQRAGETSKAPLREVWQWKRAI
ALVFFIITLHSSIFYLVLTFASTYMSSILKFDSGTTLLYVFVASLSAAVVMPFGGAFTDK
YGRKPFLLVVGTLATLAMYWLFKSAPTATPASFIYPLMTVAILFGLYASSTYALMSELLP
TRIRSTGIAVAYNIPVAVFGGSAPLISTWLIKVTGDITSPWYFYIGTGVVSLIALVLLRK
EDFVACASPAEKPNVGRADLALAPTP