Protein Info for AO353_05485 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): Fructose-specific PTS system, I, HPr, and IIA components
Rationale: Specifically important for fructose utilization. The enzyme II B/C components of this PTS system are in AO353_05475 which is cofit and is correctly annotated. AO353_05485 is 73% identical to PA3562, which is also part of a fructose-specific system (PMC:PMC2542419)
Original annotation: PTS fructose transporter subunit IIA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 953 PF00359: PTS_EIIA_2" amino acids 4 to 140 (137 residues), 96.6 bits, see alignment E=3.2e-31 TIGR01003: phosphocarrier, HPr family" amino acids 285 to 365 (81 residues), 48.5 bits, see alignment E=6.8e-17 PF00381: PTS-HPr" amino acids 285 to 366 (82 residues), 61.6 bits, see alignment E=1.6e-20 PF05524: PEP-utilisers_N" amino acids 404 to 519 (116 residues), 78.9 bits, see alignment E=8.9e-26 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 406 to 951 (546 residues), 490.1 bits, see alignment E=7.8e-151 PF00391: PEP-utilizers" amino acids 544 to 616 (73 residues), 77.8 bits, see alignment 1e-25 PF02896: PEP-utilizers_C" amino acids 644 to 925 (282 residues), 361.8 bits, see alignment E=6.3e-112

Best Hits

KEGG orthology group: K11189, phosphocarrier protein (inferred from 92% identity to pfo:Pfl01_0793)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W2F2 at UniProt or InterPro

Protein Sequence (953 amino acids)

>AO353_05485 Fructose-specific PTS system, I, HPr, and IIA components (Pseudomonas fluorescens FW300-N2E3)
MLELTIEQISMGQSAVDKAAALHLLADTLVNDGLVAEGYLSGLQAREAQGSTFLGQGIAI
PHGTPDTRDLVHTTGVRLLQFPEGVDWGDGHIVYLAIGIAAKSDEHLRLLQLLTRALGET
DLGQALRRASSAEALLKLLQGAPQELALDAQMIGLGVSADDFEELVWRGARLLRQADCVS
NGFSAVLQQVEALPLGDGLWWLHSEQTVKRPGLAFVTPDKPIRYLGQPLSGLFCLASLGE
AHQALLERLCALLIEGRGHELGRATSRRAVLEVLGGELPADWPSARIALANAHGLHARPA
KILAQLAKSFEGEIRIRIVDGQDSAVSVKSLSKLLSLGARRGQVLELIAEPSIAADALPA
LLRAIEEGLGEDIEPLPTVSAQSEVIDEITDVVVAPASGCVIQAVAAAPGIAIGPAHIQV
LQAIDYPLRGESTAIERERLKTSLADVRRDIEGLIQRSKAKAIREIFITHQEMLDDPELT
DEVDTRLKQGESAEAAWMAVIDAAARQQESLQDALLAERAADLRDIGRRVLAQLCGIETP
SEPDQPYILVMDEVGPSDVARLDPTRVAGILTARGGATAHSAIVARALGIPALVGAGAAV
LRLASGTPLLLDGQRGRLHVDADAATLQRAAEERDNREQRLQAAAAQRHQPALTTDGHAV
EVFANIGESAGVVSAVEQGAEGIGLLRTELIFMAHQQAPDEATQEVEYRRVLDGLAGRPL
VVRTLDVGGDKPLPYWPIAKEENPFLGVRGIRLTLQRPQIMEAQLRALLRAADNRPLRIM
FPMVGSVDEWRQARDMTERLRLEIPVADLQLGIMIEVPSAALLAPVLAKEVDFFSVGTND
LTQYTLAIDRGHPTLSAQADGLHPAVLQLIDITVRAAHAHGKWVGVCGELAADPLAVPVL
VGLGVDELSVSARSIGEVKARVRELSLAQVKHLAQLALAVGSANEVRALVEAL