Protein Info for AO353_05465 in Pseudomonas fluorescens FW300-N2E3

Annotation: flavodoxin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03929: PepSY_TM
  • amino acids 7 to 362 (356 residues), 224.5 bits, E=4.1e-70
• PF00258: Flavodoxin_1
  • amino acids 388 to 511 (124 residues), 100.4 bits, E=1.6e-32
• PF00175: NAD_binding_1
  • amino acids 712 to 817 (106 residues), 50 bits, E=6.6e-17

Best Hits

KEGG orthology group: K00380, sulfite reductase (NADPH) flavoprotein alpha-component [EC: 1.8.1.2] (inferred from 84% identity to pfo:Pfl01_0797)

Predicted SEED Role

"Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB"

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Sequence Analysis Tools

See A0A0N9WFY8 at UniProt or InterPro

Protein Sequence (843 amino acids)

>AO353_05465 flavodoxin (Pseudomonas fluorescens FW300-N2E3)
VLKKTLFQLHWFFGISAGLVLALMGITGAAVSFQDEILRALNPQVLQVEKQVAGVLPPAD
LVAKVEAAEGKKVAMLWVETESGFAARVFFTPPPGERRGQMRYVDPYTGDYMGDAVGQDF
FGLMLQLHRFLAMGDTGRQITGACTLILIFFCLSGLYLRWPRQVASWRAWLTLDWAKKGR
SFNWDLHSVAGTWCLLVYLLAALTGLSWSYEWYNKGLSKLLSDAPQNERVRQGRGAPPPA
GPLPTADYNAIWSSIYSAAGPGLSAYNVRMPPVAGQPATVFYLLKNSPHDRALNQITLDP
ATGVISRHDRYGDKSFKAQLLTSIYALHVGSYFGLVGRIVLTLASLTMPLFFITGWLLYL
DRRRKQRQIKNARKELMHTTSDVPAWLIGFASQSGFAEQLAWQTASQLQAAGLPVKVQPL
AAVSEQDLRESSNALFVVSTFGDGEAPDSARGFERKVLGRALSLSSLNYAVLGLGDRQYQ
HFCGFAKRLHSWLSEHGGTTLFAPVEVDSGDPYALRHWQQQLGQLTGQPPVDTWKAPSYN
NWTLSKRELLNPDSSGADVYLLGLTAPDTSSWLAGDLVEVLPRNCRGAIEQFLEGLGIAG
NSRVQINGVQETLEHALASRQLPENRVHLVGLHAQALVDALVPLAMREYSIASIAADGVL
ELLVRQECHADGRLGIGSGWLTEYAPLGSAISLRVRRNSGFHLPATPVPMILLGNGTGLA
GLRSLLKARIADGQTRNWLLFGERNREHDFLCRDELEGWLIESDLERLDLAFSRDQAEKI
YVQDRLRQRAVELKKWLADGASIYICGSLQGMATGVDQVLNEVLGVDEVERLIEQGRYRR
DVY