Protein Info for AO353_05460 in Pseudomonas fluorescens FW300-N2E3

Annotation: ligand-gated channel protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 776 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details PF07715: Plug" amino acids 79 to 177 (99 residues), 82.6 bits, see alignment E=2.8e-27 TIGR01783: TonB-dependent siderophore receptor" amino acids 81 to 776 (696 residues), 290.5 bits, see alignment E=1.5e-90 PF00593: TonB_dep_Rec" amino acids 293 to 745 (453 residues), 211.6 bits, see alignment E=4.1e-66

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 88% identity to pfo:Pfl01_0798)

Predicted SEED Role

"Ferrichrome-iron receptor" in subsystem Iron acquisition in Vibrio or Transport of Iron

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VRK0 at UniProt or InterPro

Protein Sequence (776 amino acids)

>AO353_05460 ligand-gated channel protein (Pseudomonas fluorescens FW300-N2E3)
MSRQLPQSPVSSPRLLASAIGVAITASSAAHMAYAAEKTDDKAPRNAIALDATSVTGEAQ
DSTSYQVEKASSPKYTAPLIDTPRSVTVIPQQVLKDTGALNMQDALRTVPGITFGAGEGG
NPQGDRPFIRGFDAQGDTYLDGVRDTGSQSREIFAVESIEVSKGPNSAIGGRGAAGGSIN
LVSKKAHLGDSFNGGFTWGSDQTQRYTLDSNYQFSDTAAGRLNLLSHESNVAGRDKVDYD
RWGIAPSLAFGLGTDTRVNLDYYHLQSNDLPDSGIPYTVPASGKTADRTKSNPDKPYAGG
DSSNFYGLTDRDFRKTRTDTATFAIEHDLSDALTIKNTLRHGTSMQDYILTQPDDSKGNV
NNGSVWRRANTRVSNVATTTNQTDLFGNFYIAGFKNSFSTGVEFTREEGEKSSYNVNTDT
TPGSGPADKPSSNCTPSMIGASSGYNCTSLANPNPNDPWNGAISRNYAGTDTTANTRALY
VFDTLELSPQWLVNMGLRYDHFDTKYKTFTAAGATTAKGADTSEFVTGQLGVVYKPAENG
SIYASYATSATPPGNTLGEGMDGNPLSGTKDRNGNLLSSDMEPETTKNYEVGTKWDLLND
RLSLTAALFRTEKENARVQVDTTSYANAGKTRVQGIELSANGKITDKWQVFAGYAYMDSE
QVDGGPLNHATDGNQLPNTPKNSASLWSTYNVTPKLTLGGGAFYVDDVFGSVANTTMVNS
YVRYDAMASYKLTKNVDLQLNLQNLTDVTYYDKAFATHFANQAPGRTALLSTNFHF