Protein Info for AO353_05310 in Pseudomonas fluorescens FW300-N2E3

Annotation: phospholipase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

No protein families (PFam or TIGRFam), signal peptides, or transmembrane helices were found in this protein.

Best Hits

KEGG orthology group: None (inferred from 75% identity to pfl:PFL_0888)

Predicted SEED Role

"Phosphatidylcholine-hydrolyzing phospholipase C (EC 3.1.4.3)" (EC 3.1.4.3)

MetaCyc Pathways

phospholipases (1/5 steps found)
plasmalogen degradation (2/8 steps found)
2-arachidonoylglycerol biosynthesis (1/7 steps found)
plasmalogen biosynthesis I (aerobic) (2/16 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W2A3 at UniProt or InterPro

Protein Sequence (539 amino acids)

>AO353_05310 phospholipase (Pseudomonas fluorescens FW300-N2E3)
MSGLHTQSQENLNHDDRIIADLDQLFSERGIAISDDGDHLILLTHSQHSPKHHTPGLAGK
SLNTEPAPRFEGGEHTAIGDATLLRFVKDAPAIAAWQVPLHLPNGLALSYGQIVSLGGDF
YGIPDQPICDGATPVDRIQRFTAAFNSLAVLPASRAEAGLILGVMQKEIAAANQAIRDGK
QPHEAYDALGDTLSEEWNKITGGGSFVSALFPLGRYLKLAANNADHFGEWALLAYIAGHT
AALQQAVLAHNSGDEKQLELAYAMNAFADHYLTDLFSAGHLRIPRKQLAAAVTPSDLGSL
ITRFMHDEDSKYGLNVNNANGDQWHAYGDKRYFDSVDSANRKQVKLAVQRSADEVFDTFL
SGSLPAPSSYVALKYLPDLNSVKKPAGNFSGLFVLDGNKVLRRSDVNNLNDSSKIDNWWG
WSTYLLLKDYTPNKPAGYLESPATAPTIRADGWQSQTPGEPNWLPGNAVRYAFSYTNGLN
ESYIGPWSNYAELSERFQPTLNVPGNGASRNIFRQFRGGSPELIGSIDAHTSTFIDRNA