Protein Info for AO353_05295 in Pseudomonas fluorescens FW300-N2E3

Annotation: glucarate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 48 to 70 (23 residues), see Phobius details amino acids 79 to 97 (19 residues), see Phobius details amino acids 103 to 122 (20 residues), see Phobius details amino acids 145 to 167 (23 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details amino acids 245 to 268 (24 residues), see Phobius details amino acids 281 to 306 (26 residues), see Phobius details amino acids 318 to 337 (20 residues), see Phobius details amino acids 343 to 363 (21 residues), see Phobius details amino acids 375 to 398 (24 residues), see Phobius details amino acids 404 to 425 (22 residues), see Phobius details PF07690: MFS_1" amino acids 17 to 305 (289 residues), 175 bits, see alignment E=2.1e-55 TIGR00893: D-galactonate transporter" amino acids 19 to 426 (408 residues), 528.5 bits, see alignment E=5.3e-163

Best Hits

Swiss-Prot: 67% identical to GUDP_BACSU: Probable glucarate transporter (gudP) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 91% identity to pba:PSEBR_a819)

MetaCyc: 62% identical to galactarate/D-glucarate transporter GarP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-203; TRANS-RXN0-204; TRANS-RXN0-523

Predicted SEED Role

"D-galactarate permease" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VKW8 at UniProt or InterPro

Protein Sequence (453 amino acids)

>AO353_05295 glucarate transporter (Pseudomonas fluorescens FW300-N2E3)
MQATKPTHVRYLILLMLFLVTTINYADRATIAIAGSSLQKDLGIDAVTLGYIFSAFGWAY
VAGQIPGGWLLDRFGSKKVYALSIFTWSLFTVLQGYVGEFGVSTAIVALFVLRFLVGLAE
APSFPGNARIVAAWFPTAERGTASAIFNSAQYFATVIFAPLMGWIVYSFGWQHVFMVMGG
IGIVFSLIWLKIIHSPRQHPMINDSEFKHIADNGGLVDMDQDRGKRSDGPKWDYVRQLLS
NRMMLGVYLGQYCINGITYFFLTWFPVYLVQERGMTILKAGFIASLPAICGFIGGVLGGL
ISDYLLRKGHSLTFARKAPIIGGLLLSTSIVACNYVNIEWIVVGFMALAFFGKGVGALGW
AVVSDTSPKQIAGLSGGLFNMFGNIASITTPIVIGYIISTTGSFKWALVFVGCNALVAVF
SYLVIVGPIKRVVLKEPPANDSQLTNKLSQAHS