Protein Info for AO353_05260 in Pseudomonas fluorescens FW300-N2E3
Annotation: glutamyl-tRNA amidotransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to GATA_PSEPF: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 96% identity to pfl:PFL_0894)MetaCyc: 54% identical to glutamyl-tRNA(Gln) amidotransferase, A subunit (Synechococcus elongatus PCC 7942 = FACHB-805)
Glutaminyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7]; Asparaginyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7, 6.3.5.6]
Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamine degradation I (1/1 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9VQP5 at UniProt or InterPro
Protein Sequence (483 amino acids)
>AO353_05260 glutamyl-tRNA amidotransferase (Pseudomonas fluorescens FW300-N2E3) MHQLTLAEIARGLADKKFSSEELTKVLLARIAQLDPQLNSFISLTEDLALSQAKAADVRR ANGESGALLGAPIAHKDLFCTQGIRTSCGSKMLDNFKAPYDATVVAKLAAAGAVTLGKTN MDEFAMGSANESSYYGAVKNPWNLEHVPGGSSGGSAAAVAARLLPAATATDTGGSIRQPA AFTNLTGLKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCAILLQGMAGFDPKDSTSIDE PVPDYSAGLNGSLQGLRIGVPKEYFSAGLDPRIAELIHNSVKELEKLGAVVKEISLPNMQ HAIPAYYVIAPAEASSNLSRFDGVRFGYRCENPKDLTDLYKRSRGEGFGPEVQRRIMVGA YALSAGYYDAYYLKAQKIRRLVKNDFMAAFNEVDIILGPTTPNPAWKLGAKNSDPVAAYL EDVYTITANLAGLPGLSMPAGFVDGLPVGVQLLAPYFQEGRLLNVAHQYQLNTDWHTRTP TGF