Protein Info for AO353_05205 in Pseudomonas fluorescens FW300-N2E3

Annotation: peptidase PmbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 250 to 262 (13 residues), see Phobius details PF01523: PmbA_TldD_1st" amino acids 34 to 98 (65 residues), 50.1 bits, see alignment E=3.9e-17 PF19290: PmbA_TldD_2nd" amino acids 126 to 232 (107 residues), 79.9 bits, see alignment E=3.1e-26 PF19289: PmbA_TldD_3rd" amino acids 239 to 447 (209 residues), 234.8 bits, see alignment E=1e-73

Best Hits

Swiss-Prot: 50% identical to PMBA_HAEIN: Metalloprotease PmbA homolog (pmbA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03592, PmbA protein (inferred from 94% identity to pfs:PFLU0872)

Predicted SEED Role

"TldE protein, part of TldE/TldD proteolytic complex"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WEW4 at UniProt or InterPro

Protein Sequence (448 amino acids)

>AO353_05205 peptidase PmbA (Pseudomonas fluorescens FW300-N2E3)
MSAVESVGPQALPALQEQVEQIIAEARRQGASACEVAVSLEQGLSTTVRQREVETVEFNR
DQGFGITLYVGQRKGSASTSASGPEAIRETVAAALAIAKHTSEDEHSGLADAALMAKDLP
DFDLFHEWDITPEQAIERALACEAAAFATDSRIKNADGTTLSTHQGCRVYGNSHGFIGGY
ASTRHSLSCVMIAEADGQMQRDYWYDVNRQGNLLADPLSIGQRAAQRAASRLGARPVPTC
EVPVLFSAELAGGLFGSFLGAISGGNLYRKSSFLEGALGQKLFPEWLTIDERPHLMRALG
SSAFDGDGLATYAKPFVEKGELVSYVLGTYSGRKLGMPSTANAGGVHNLFVTHGDEDQAA
LLRRMGRGLLVTELMGQGLNMVTGDYSRGAAGFWVENGEIQFAVQEVTIAGNMRDMFKQI
IAVGNDLELRSNIRTGSVLIERMTVAGS