Protein Info for AO353_04705 in Pseudomonas fluorescens FW300-N2E3

Annotation: poly(beta-D-mannuronate) C5 epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 539 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details PF05048: NosD" amino acids 285 to 387 (103 residues), 32.5 bits, see alignment E=5.9e-12 amino acids 373 to 439 (67 residues), 29.1 bits, see alignment E=6.7e-11 PF13229: Beta_helix" amino acids 295 to 439 (145 residues), 53 bits, see alignment E=3.1e-18 TIGR03804: parallel beta-helix repeat" amino acids 373 to 411 (39 residues), 21.1 bits, see alignment 9.6e-09

Best Hits

Swiss-Prot: 82% identical to ALGG_PSEFL: Mannuronan C5-epimerase (algG) from Pseudomonas fluorescens

KEGG orthology group: None (inferred from 88% identity to pfo:Pfl01_0955)

MetaCyc: 68% identical to mannuronan C5 epimerase (Pseudomonas aeruginosa)
RXN-9839 [EC: 5.1.3.37]; TRANS-RXN-269 [EC: 5.1.3.37]

Predicted SEED Role

"Poly(beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-)" in subsystem Alginate metabolism (EC 5.1.3.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.- or 5.1.3.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W1Z8 at UniProt or InterPro

Protein Sequence (539 amino acids)

>AO353_04705 poly(beta-D-mannuronate) C5 epimerase (Pseudomonas fluorescens FW300-N2E3)
MRAAKGVPQMNHPALKGSLSLLAAAMLLASSAVFANVEPVAPVPKGPQATVAKGLQQAKT
YTVSSAPTESLELAAPKLPDLSGYTAQAVAAKIVRSKAGKVSTRRMMQEDALKDFIGGDN
KMAEWVVRQHGIPQAIFIDDGYINLKDLAQKLPKQYFSETSPGVFLARLPIVVGQKGILE
IDQQTQELRLSEEAGSFMINDGQLFVRDSKITGWREKDNGPATFRSPKDFRPFLLSWGGT
QTYIVNSKLASFGYSNSKSYGVSISQYTPNMAKVLKRPEPTGWIIGSEFSDMWYGFYCYE
TRDFVVKGNTYRDNIVYGIDPHDRSHGLIIADNTVHGTKKKHGIIISREVNDSFIFNNRS
YDNHLSGLVIDRNSVNNLIAYNEIYRNHTDGITLYESADNLLWGNKVISNQRHGIRIRNS
VNIRLYENVAMNNGLTGVYGHIKDLTDTDRDIKLDPFDAEVSLIVVGGELAANGSGPLSI
DSPLSVELYRVSMLAPTKSSGISFSGILGERQDEILDLLVRQQKAVLIDPVERQTEMRD