Protein Info for AO353_04685 in Pseudomonas fluorescens FW300-N2E3

Annotation: glycosyl transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 49 to 73 (25 residues), see Phobius details amino acids 364 to 390 (27 residues), see Phobius details amino acids 396 to 418 (23 residues), see Phobius details amino acids 429 to 447 (19 residues), see Phobius details amino acids 475 to 495 (21 residues), see Phobius details PF13641: Glyco_tranf_2_3" amino acids 89 to 345 (257 residues), 139 bits, see alignment E=2.4e-44 PF13632: Glyco_trans_2_3" amino acids 186 to 412 (227 residues), 94.6 bits, see alignment E=7.6e-31

Best Hits

Swiss-Prot: 90% identical to ALG8_PSESM: Glycosyltransferase alg8 (alg8) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a4591)

MetaCyc: 83% identical to mannuronosyl transferase catalytic subunit (Pseudomonas aeruginosa)
Alginate synthase. [EC: 2.4.1.33]

Predicted SEED Role

"Alginate biosynthesis protein Alg8" in subsystem Alginate metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VR05 at UniProt or InterPro

Protein Sequence (496 amino acids)

>AO353_04685 glycosyl transferase (Pseudomonas fluorescens FW300-N2E3)
MQIMHRLKHRLLQAAGWLFYLSLLMGIAMALPVSTFDSQSKDFIFLIGAVGIWRYSMGAT
HFLRGMLFLYVVYPHLRRKVRKLGKTADPSHVFLMVTSFRIDALTTAQVYSSVIREAIEC
ELPTTVVCSIVEMSDELLVKSLWAKMNPPDRVKLDFVRIPGTGKRDGLAFGFRAISRHLP
DDRAVVAVIDGDTVLAEGVVRKTVPWFQLFGNVGGLTTNEFCEVRGGYIMSEWHKLRFAQ
RHINMCSMALSKRVLTMTGRMSVFRASVVTDPDFIADVESDSLQHWRLGRFKFLTGDDKS
SWFSLMRLGYDTFYVPDAAINTVEHPPEKSFIKASRKLMFRWYGNNLRQNSRALGLGMKR
LGLFTSVVLFDQRVSMWTSLLGLTVALIASFKYGTAFILVYLLWIGITRLILTLLLSCSG
HKIGPAYPAILYYNQIVGALVKIYVFFRLDQQSWTRQDTKLTRDLASFQRWFNTWSSRTM
TFSAGSIFVAVLLLMV