Protein Info for AO353_04550 in Pseudomonas fluorescens FW300-N2E3

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 948 transmembrane" amino acids 501 to 523 (23 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 928 (928 residues), 1197.3 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 628 (615 residues), 512.8 bits, see alignment E=1.7e-157 PF09334: tRNA-synt_1g" amino acids 38 to 89 (52 residues), 22.1 bits, see alignment 1.5e-08 PF13603: tRNA-synt_1_2" amino acids 243 to 304 (62 residues), 31.5 bits, see alignment 3.2e-11 PF08264: Anticodon_1" amino acids 669 to 821 (153 residues), 147.1 bits, see alignment E=9.1e-47 PF10458: Val_tRNA-synt_C" amino acids 883 to 945 (63 residues), 68.2 bits, see alignment 1.5e-22

Best Hits

Swiss-Prot: 67% identical to SYV_COLP3: Valine--tRNA ligase (valS) from Colwellia psychrerythraea (strain 34H / ATCC BAA-681)

MetaCyc: 63% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WEI8 at UniProt or InterPro

Protein Sequence (948 amino acids)

>AO353_04550 valine--tRNA ligase (Pseudomonas fluorescens FW300-N2E3)
MDKTYQPHAIETSWYNTWESENYFAPQGAGESYTIMIPPPNVTGSLHMGHGFNNAIMDAL
IRFRRMQGRNTLWQPGTDHAGIATQMLVERQLEAKGQNRHDLGREKFLEKVWEWKDESGG
NISRQIRRLGSSVDWSRERFTMDDGLSEAVKEAFVRLHEDGLIYRGKRLVNWDTKLHTAI
SDLEVENHDEKGFLWNLKYPLADGAKTAEGNDYLIVATTRPETMLGDAAVAVNPNDERYK
ALIGKYVVLPLVGRRIPIIADDYCDPEFGTGCVKITPAHDFNDYEVGKRHNLPLLNIFDK
NAEVLPAAQVFNLDGSVNEAIDGTLPAEYAGLNRFQARKEIVAAFEAAGLLVSVDDHALK
VPKGDRSGTIIEPWLTDQWYVSTKPLAEPAIAAVEDGRIQFVPKQYENMYFSWMRDIQDW
CISRQLWWGHRIPAWYDESGKVYVGRDEAEVRAKHNLGPDVALQQDNDVLDTWFSSGLWT
FSTLGWPQQTEFLKKFHSTDVLVTGFDIIFFWVARMIMLTMHLVKNEDGTPQVPFKTVYV
HGLVRDGQGQKMSKSKGNVLDPLDIIDGIELEALVEKRTSGLMQPKLAKKIEKQTREEFA
DGIASYGTDALRFTFCSLASTGRDIKFDMGRVEGYRNFCNKIWNAARYVLDKGEDCGQNG
EAYELSLADRWIISQLQRTEAEVTRQLDQFRFDLAAQALYEFIWNQYCDWYLELSKPVLW
DENAAVERQRGTRRTLVRVLEVALRLAHPFMPFITEEIWQRIAPLAGVQGKTIMLQPWPV
AVEARIDQAAEDDIEWLKGLMLGTRNIRGEMNIGPGKPLPLFLKNVNTEDQRRLTENEVL
LKKLAKLESITVLAAGEEAPLSATALVGEMEVLVPMAGLIDKNAELARLDKEIQRLQGEV
QRVGGKLSNAGFVDKAPADVIEKERAKLAEAEQALGRLAEQHARIASL