Protein Info for AO353_04510 in Pseudomonas fluorescens FW300-N2E3

Annotation: ribonuclease III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 229 TIGR02191: ribonuclease III" amino acids 8 to 220 (213 residues), 232.1 bits, see alignment E=2.7e-73 PF14622: Ribonucleas_3_3" amino acids 18 to 140 (123 residues), 130.4 bits, see alignment E=7.2e-42 PF00636: Ribonuclease_3" amino acids 37 to 127 (91 residues), 89.7 bits, see alignment E=3e-29 PF00035: dsrm" amino acids 155 to 220 (66 residues), 38.9 bits, see alignment E=1.6e-13

Best Hits

Swiss-Prot: 96% identical to RNC_PSEU2: Ribonuclease 3 (rnc) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K03685, ribonuclease III [EC: 3.1.26.3] (inferred from 95% identity to pfo:Pfl01_0994)

MetaCyc: 55% identical to RNase III (Escherichia coli K-12 substr. MG1655)
Ribonuclease III. [EC: 3.1.26.3]

Predicted SEED Role

"Ribonuclease III (EC 3.1.26.3)" in subsystem RNA processing and degradation, bacterial or Two cell division clusters relating to chromosome partitioning (EC 3.1.26.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.3

Use Curated BLAST to search for 3.1.26.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VQW9 at UniProt or InterPro

Protein Sequence (229 amino acids)

>AO353_04510 ribonuclease III (Pseudomonas fluorescens FW300-N2E3)
VSVSLSRLERQLGYTFKDQELMVLALTHRSFAGRNNERLEFLGDAILNFVAGEALFDRFP
LAREGQLSRLRARLVKGETLAVLARGFDLGEYLRLGSGELKSGGFRRESILADALEALIG
AIYLDAGMDMARERVLAWLTSEFDSLTLVDTNKDPKTRLQEFLQSRACELPRYEVVDIQG
EPHCRTFFVECEITLLNEKSRGQGVSRRIAEQVAAAAALIALGVENGHD