Protein Info for AO353_04460 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF)
Rationale: Specifically important for NAG utilization. (SEED_correct)
Original annotation: PTS N-acetyl-D-glucosamine transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 838 PF00358: PTS_EIIA_1" amino acids 9 to 133 (125 residues), 144.9 bits, see alignment E=2.5e-46 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 11 to 130 (120 residues), 127.4 bits, see alignment E=4.4e-41 PF00381: PTS-HPr" amino acids 175 to 251 (77 residues), 71.5 bits, see alignment E=1.3e-23 TIGR01003: phosphocarrier, HPr family" amino acids 175 to 251 (77 residues), 64.1 bits, see alignment E=1.3e-21 PF05524: PEP-utilisers_N" amino acids 281 to 399 (119 residues), 79.9 bits, see alignment E=4.5e-26 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 308 to 828 (521 residues), 468.7 bits, see alignment E=3.6e-144 PF00391: PEP-utilizers" amino acids 423 to 496 (74 residues), 63.1 bits, see alignment E=3.9e-21 PF02896: PEP-utilizers_C" amino acids 522 to 807 (286 residues), 336.9 bits, see alignment E=2.4e-104

Best Hits

KEGG orthology group: K11189, phosphocarrier protein (inferred from 81% identity to pfo:Pfl01_1004)

Predicted SEED Role

"PTS system, glucose-specific IIA component / Phosphotransferase system, phosphocarrier protein HPr / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WFD8 at UniProt or InterPro

Protein Sequence (838 amino acids)

>AO353_04460 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (Pseudomonas fluorescens FW300-N2E3)
MHNNNKDLTLSAPLSGPVLTLAKVPDPVFASGAMGDGIAIDPLNNTLHAPCAGVVVHVAR
TGHAVTLRADNGAELLLHLGLDTVELQGEGFSMLVKEGTRVSNGQALLRFDLDQVAQGCK
SLVSLLVLTNSEDFQVLPITLKSVKVGEPLLHIVPRTTHSAQVEADSSGAEVHGHIRIIH
RGGLHARPAALIRQTAHLFNSKSQLHFAGKSASCDSLIGLMGLGIGEQDEVQVSCKGADA
KAALQALLNALSTAVNDDSHAAAPTPIAQRTRTAEAGVLNGVCAAPGLVGGPLFQLAAIP
LPEDTGKHNAEEQLQALDRALEQVRSEIRETLSHAKKHKHTEEEQIFAAHLALLEDPALL
EAAIQSIDQGSAATHAWSQSIEAQCEVLQQLGNPLLAERANDLRDLRQRVLRALLGQDWH
YDVPAGAIVAAHELTPSDLLQLSQQGVAGLCMAEGGATSHVAILARGKGLPCLVALSASL
LQQPQGQSVVLDADGGRLELTPDSQRLEQVAQAQREHLQRRERQQAQAHTPAHTRDGLRI
EVAANVASSNEAADALKGGADGVGLLRTEFLFVDRQTAPDEQEQRQAYQAVLDAMGDKSV
IIRTIDVGGDKQLDYLPLPAEANPVLGLRGIRMAQVRPELLDQQLRALLQVSPLQRCRIL
LPMVTEVDELLYIRQRLDALCAELALTQRLELGVMIEVPAAALLAEQLAEHADFLSIGTN
DLSQYTLAMDRDHAGLAARVDALHPALLRLIAQTCIGAAKHQRWVGVCGALASDPLATPV
LIGLGISELSVSPPQVGEIKERVRQLDAADCRRFSATLLNLSSATAVRHACHQHWPLS