Protein Info for AO353_04105 in Pseudomonas fluorescens FW300-N2E3

Annotation: argininosuccinate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00764: Arginosuc_synth
  • amino acids 6 to 167 (162 residues), 221.5 bits, E=6.5e-70
• TIGR00032: argininosuccinate synthase
  • amino acids 6 to 399 (394 residues), 474.5 bits, E=1.7e-146
• PF20979: Arginosuc_syn_C
  • amino acids 178 to 396 (219 residues), 268.7 bits, E=3.8e-84

Best Hits

Swiss-Prot: 99% identical to ASSY_PSEFS: Argininosuccinate synthase (argG) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01940, argininosuccinate synthase [EC: 6.3.4.5] (inferred from 99% identity to pfl:PFL_4853)

Predicted SEED Role

"Argininosuccinate synthase (EC 6.3.4.5)" in subsystem Arginine Biosynthesis extended (EC 6.3.4.5)

MetaCyc Pathways

• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• nitric oxide biosynthesis II (mammals) (2/3 steps found)
• urea cycle (3/5 steps found)
• canavanine biosynthesis (2/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.5

Sequence Analysis Tools

See A0A0P8X461 at UniProt or InterPro

Protein Sequence (405 amino acids)

>AO353_04105 argininosuccinate synthase (Pseudomonas fluorescens FW300-N2E3)
MADVNKVVLAYSGGLDTSVILKWLQDTYNCEVVTFTADLGQGEEVEPARAKAQAMGVKEI
YIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIAKRLIEIANETGADAISHGAT
GKGNDQVRFELGAYALKPGVKVIAPWREWDLLSREKLMDYAAKHAIPIERHGKKKSPYSM
DANLLHISYEGGVLEDTWTEHEEDMWRWTVSPENAPDKPQYLELTYRNGDIVALDGVEMT
PATVLATLNRIGGEHGIGRLDIVENRYVGMKSRGCYETPGGTIMLRAHRAIESITLDREV
AHLKDELMPKYASLIYTGYWWSPERLMLQQMIDASQAHVNGVVRLKLYKGNVIVTGRKSD
ESLFDANIATFEEDGGAYNQADAAGFIKLNALRMRIAANKGRKLF