Protein Info for AO353_04080 in Pseudomonas fluorescens FW300-N2E3

Annotation: NADH:quinone oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 196 transmembrane" amino acids 23 to 42 (20 residues), see Phobius details amino acids 55 to 80 (26 residues), see Phobius details amino acids 86 to 105 (20 residues), see Phobius details amino acids 117 to 135 (19 residues), see Phobius details amino acids 165 to 185 (21 residues), see Phobius details PF02508: Rnf-Nqr" amino acids 7 to 183 (177 residues), 70.2 bits, see alignment E=1.2e-23

Best Hits

KEGG orthology group: K03613, electron transport complex protein RnfE (inferred from 51% identity to pfl:PFL_4849)

Predicted SEED Role

"Electron transport complex protein RnfE" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WEA3 at UniProt or InterPro

Protein Sequence (196 amino acids)

>AO353_04080 NADH:quinone oxidoreductase (Pseudomonas fluorescens FW300-N2E3)
MNQSSMLQNSLILTPLIGASDSLVKALGLSLLSLLVIGVYGLKMSVLRPRIVPSMQLITC
IALAATLTSLATLALQAWAFELHQQLGMYAGLIALQCIVLEYSGFFTQASMIDRTRLSSL
FFGLMVVLGVLREALGNGSFASHVSWLSGIIDSSGQGWLFAADGGIRLAILAPGGFILLG
LLIAAKQAWPGASTSH