Protein Info for AO353_04055 in Pseudomonas fluorescens FW300-N2E3

Annotation: methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 683 TIGR00398: methionine--tRNA ligase" amino acids 7 to 535 (529 residues), 580.5 bits, see alignment E=3.6e-178 PF09334: tRNA-synt_1g" amino acids 7 to 395 (389 residues), 522.6 bits, see alignment E=1.7e-160 PF00133: tRNA-synt_1" amino acids 11 to 106 (96 residues), 33.7 bits, see alignment E=5.4e-12 PF19303: Anticodon_3" amino acids 417 to 517 (101 residues), 25.8 bits, see alignment E=2.7e-09 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 561 to 683 (123 residues), 140.8 bits, see alignment E=2.6e-45 PF01588: tRNA_bind" amino acids 587 to 681 (95 residues), 86.9 bits, see alignment E=2.4e-28

Best Hits

Swiss-Prot: 95% identical to SYM_PSEFS: Methionine--tRNA ligase (metG) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 96% identity to pba:PSEBR_a4380)

MetaCyc: 64% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W7U2 at UniProt or InterPro

Protein Sequence (683 amino acids)

>AO353_04055 methionine--tRNA ligase (Pseudomonas fluorescens FW300-N2E3)
MSEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCTYVCADDAHGSAIML
RAEKEGITPEQLIANVQAEHSADFAEFLVDFDNFHSTHAEENRELSSQIYLKLRDAGHIA
TRSITQYFDPEKKMFLADRFIKGTCPKCGTEDQYGDNCEKCGATYAPTDLKDPKSAISGA
TPVLKDSQHFFFKLPDFQQMLQEWTRSGTLQDAVANKIAEWLDVGLQQWDISRDAPYFGF
EIPDEPGKYFYVWLDAPIGYMASFKNLCARRPELDFDAYWAKDSTAELYHFIGKDIVNFH
ALFWPAMLEGAGYRKPTGINVHGYLTVNGQKMSKSRGTFIKARTYLDHLSPEYLRYYYAS
KLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIHKGNAGVLVAGNAAPELTDAF
LAAAPSIAEAYEARDFARAMREIMGLADRANAWIADKAPWSLNKQEGKQDEVQAICALGV
NLFRQLVIFLKPVLPLLAADAEAFLNVAPLTWNDHATLLSNHQLNEFKPLMTRIDPVKVQ
AMTDASKEDLAASQTDTGDAAPQGNGELAKDPLSPEIEFDTFAAVDLRVALILKAEAVEG
ADKLLRLTLDIGDEQRNVFSGIKSAYPDPAKLEGRLTMMIANLKPRKMRFGISEGMVMAA
GPGGEEIYLLSPDSGAKPGQRIK