Protein Info for AO353_03995 in Pseudomonas fluorescens FW300-N2E3

Annotation: RND transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 44 to 366 (323 residues), 253.6 bits, see alignment E=1.2e-79 PF13533: Biotin_lipoyl_2" amino acids 70 to 114 (45 residues), 26.7 bits, see alignment 7.7e-10 PF16576: HlyD_D23" amino acids 71 to 269 (199 residues), 118.8 bits, see alignment E=4.2e-38 PF13437: HlyD_3" amino acids 166 to 266 (101 residues), 71.7 bits, see alignment E=1.6e-23

Best Hits

KEGG orthology group: None (inferred from 82% identity to pfs:PFLU4978)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VK85 at UniProt or InterPro

Protein Sequence (383 amino acids)

>AO353_03995 RND transporter (Pseudomonas fluorescens FW300-N2E3)
MLRRRMLIMLGVVLLIILMLGGYKAFSIYKMIQTFAVPRPPVSVAVATATERPWQSRLPT
VGTLKALQGVNLSLEIAGTVKDVQFESGQKVKAGQPLVQLDSAVESALLETAQADLGLAQ
LDYGRGSQLVGSQAISKGEFDRLTAVLAKNKATVNQLKAALAKKHIVAPFSGTIGIRQVD
VGDFLPSGTVIATLQNLSSLYVDFFVPEQMVPKIALGQAVQVTVPAYPTQNFPGAISAIN
PKVESSTRNVQVRATLANPDGTLLPGMFASLQVMLPDPQPRIVVPEGAITYTLYGNSLYV
VAQKKDANGNVEKDDQGQPILIAERRFIETGERREGLVTINKGLQNGEQVVSAGQIKLDN
GTHIVVSPDKNLPAVPTSQPRTN