Protein Info for AO353_03970 in Pseudomonas fluorescens FW300-N2E3

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 683 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 153 to 182 (30 residues), see Phobius details TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 248 to 409 (162 residues), 98.4 bits, see alignment E=1.8e-32 PF00990: GGDEF" amino acids 250 to 407 (158 residues), 120.6 bits, see alignment E=5.9e-39 PF00563: EAL" amino acids 427 to 662 (236 residues), 265.9 bits, see alignment E=2.9e-83

Best Hits

KEGG orthology group: None (inferred from 96% identity to pfo:Pfl01_4487)

Predicted SEED Role

"GGDEF domain/EAL domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WDH3 at UniProt or InterPro

Protein Sequence (683 amino acids)

>AO353_03970 diguanylate cyclase (Pseudomonas fluorescens FW300-N2E3)
LKLELKNSLSVKLLRVVLLSALIVGVVLSCAQIVFDAYKTRQAVANDAQRILDMFRDPST
QAVYSLDREMGMQVIEGLFQDDAVRMASIGHPNEAMLAEKTRDLQHSHSRWLTDLILGQE
RTFTTQLVGRGPYSEYYGDLSITLDTATYGQGFIVSSVIIFISGVLRAMAMSLVLYLVYH
WLLTKPLSRIIEHLTNVNPDRPSEHKIPQLKGHEKNELGIWINTANQLLESIERNTHLRH
EAENSLLRMAQYDFLTGLPNRQQLQQQLDKILVDAGRLQRRVAVLCVGLDDFKGINEQFS
YQTGDQLLLALADRLRAHSGRLGALARLGGDQFALVQADIEQPYEAAELAQSILDDLEAA
FALDHQEIRLRATIGITLFPEDGDSTEKLLQKAEQTMTLAKTRSRNRYQFYIASVDSEMR
RRRELEKDLRDALTRNQFYLVYQPQISYRDQRVVGVEALIRWQHPEHGLVPPDLFIPLAE
QNGTIIAIGEWVLDQACRQLREWHDQGFSDLRMAVNLSTVQLHHAELPRVINNLMQMYRL
PPRSLELEVTETGLMEDISTAAQHLLSLRRSGALIAIDDFGTGYSSLSYLKSLPLDKIKI
DKSFVQDLLDDDDDATIVRAIIQLGKSLGMQVIAEGVETVEQEAYIISEGCHEGQGYFYS
KPLPARELAAYLKQAQRSNALIL