Protein Info for AO353_03600 in Pseudomonas fluorescens FW300-N2E3

Annotation: aspartate--tRNA(Asp/Asn) ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 TIGR00459: aspartate--tRNA ligase" amino acids 1 to 583 (583 residues), 811.7 bits, see alignment E=1.7e-248 PF01336: tRNA_anti-codon" amino acids 19 to 96 (78 residues), 63.5 bits, see alignment E=2.8e-21 PF00152: tRNA-synt_2" amino acids 121 to 559 (439 residues), 408.4 bits, see alignment E=4.1e-126 PF02938: GAD" amino acids 310 to 409 (100 residues), 90.9 bits, see alignment E=1e-29

Best Hits

Swiss-Prot: 98% identical to SYDND_PSEF5: Aspartate--tRNA(Asp/Asn) ligase (aspS) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 98% identity to pfl:PFL_4767)

MetaCyc: 62% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23)" (EC 6.1.1.12, EC 6.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WE17 at UniProt or InterPro

Protein Sequence (591 amino acids)

>AO353_03600 aspartate--tRNA(Asp/Asn) ligase (Pseudomonas fluorescens FW300-N2E3)
MMRSHYCGQLNESLEGQEVTLCGWVHRRRDHGGVIFLDIRDREGLAQVVFDPDRAETFAA
ADRVRSEYVVKITGKVRLRPAGAGNANMASGMIEVLGYELEVLNEAETPPFPLNEFSDVG
EETRLRYRFIDLRRPEMLEKLRLRSRMTTSIRRYLDENGFLDVETPILTRATPEGARDYL
VPSRTHAGSFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETS
FLNEAEIMGLTEGMIRNLFKEVLGLEFGEFPHMTWDEAMRRYGSDKPDLRNPLELVDVAD
QLKEVDFKVFSGPANDPKSRIAALRVPGGASMPRSKIDDYTKFVGIYGAKGLAYIKVNER
AKGVEGLQSPIVKNIPEANLNVILDRVGAVDGDIVFFGADKAKIVSEALGALRIKVGHDL
NLLTCEWAPMWVVDFPMFEENDDGSFTALHHPFTAPKCTPEELEANPATALSRAYDMVLN
GTELGGGSIRIHRKEMQQSVFRLLGISEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLV
MLMTGAQSIREVIAFPKTQSAACVMTQAPGLVDAKALRELHIRLREQPKAE