Protein Info for AO353_03580 in Pseudomonas fluorescens FW300-N2E3

Annotation: ATP-dependent DNA helicase RuvB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 353 PF05496: RuvB_N" amino acids 25 to 183 (159 residues), 272.1 bits, see alignment E=3.4e-85 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 29 to 331 (303 residues), 495.1 bits, see alignment E=3.3e-153 PF00004: AAA" amino acids 61 to 183 (123 residues), 74.2 bits, see alignment E=3.4e-24 PF17864: AAA_lid_4" amino acids 186 to 259 (74 residues), 109.8 bits, see alignment E=1.1e-35 PF05491: RuvB_C" amino acids 260 to 330 (71 residues), 85.9 bits, see alignment E=3.7e-28

Best Hits

Swiss-Prot: 96% identical to RUVB_PSEF5: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 97% identity to pfs:PFLU4913)

MetaCyc: 72% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W1D1 at UniProt or InterPro

Protein Sequence (353 amino acids)

>AO353_03580 ATP-dependent DNA helicase RuvB (Pseudomonas fluorescens FW300-N2E3)
VIEADRLIAATGGPRDREEVQDRAIRPVSLAEYIGQPTVREQMELFIQAARGRNESLDHT
LIFGPPGLGKTTLANIIAQEMGVSIKSTSGPVLERPGDLAALLTNLEPHDVLFIDEIHRL
SPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGMLTNPLRDRFGIV
QRLEFYSTADLATIVSRSANILGLPLDPEGAFEIARRARGTPRIANRLLRRVRDFAEVRA
KGHITKPIADLALNLLDVDERGFDHQDRRLLLTMIEKFDGGPVGVDSLAAAISEERHTIE
DVLEPYLIQQGYIMRTPRGRVVTRHAYLHFGLNIPSRLGEMPVPDEFLDAVDE