Protein Info for AO353_03400 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): D-mannose isomerase (EC 5.3.1.7)
Rationale: Specifically important for: D-Mannose. Often annotated as N-acylglucosamine 2-epimerase, but this is also distantly related to the D-mannose isomerase Sama_0560. The putative fructokinase AO353_25910 is also important for mannose utilization, which would complete the pathway.
Original annotation: sugar isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07221: GlcNAc_2-epim
  • amino acids 40 to 394 (355 residues), 426.4 bits, E=4.9e-132

Best Hits

Swiss-Prot: 47% identical to SQUS_SALTY: Sulfoquinovose isomerase (yihS) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 83% identity to pfo:Pfl01_4371)

Predicted SEED Role

"N-acylglucosamine 2-epimerase (EC 5.1.3.8)" in subsystem Sialic Acid Metabolism (EC 5.1.3.8)

MetaCyc Pathways

• N-acetylneuraminate and N-acetylmannosamine degradation II (1/3 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.8 or 5.3.1.7

Sequence Analysis Tools

See A0A0N9WB79 at UniProt or InterPro

Protein Sequence (419 amino acids)

>AO353_03400 D-mannose isomerase (EC 5.3.1.7) (Pseudomonas fluorescens FW300-N2E3)
MNTAPPAFSSWLNAPAHQQWLADEGLRLLAFAKASKLPEGFGNLDEHGQLPADAQAHTMN
TARMTHSFAMAHIQGLPGFAELVDHGINALNGLLRDAEFGGWFAAPDHRDGDTGKAAYLH
AFVALAASSAVIAQRPGAEALLNDAIAIIEQHFWSEEEGAMRESFNRDWSEEEPYRGANS
NMHATEAFLALADVTQDSRWLNRALRIVERVIHRHAASNDHLVVEHFDRDWQPLRDYNQA
NPADHFRPYGTTPGHGFEWSRLLLHLEAARGQAGMLTPGWLLSDAQQLFANNCLHGWDVD
GAPGIVYTLDWDNRPVVRQRLHWVHCEASAAASVLLKRTGEAQYETWYRRFWEFSDRCLI
DRIHGSWHHELDPQNRPSTEIWGGKPDLYHAWQAVLIPRLPLAPSMASALAQGSVSVLM