Protein Info for AO353_03310 in Pseudomonas fluorescens FW300-N2E3

Annotation: long-chain fatty acid--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 31 to 419 (389 residues), 319.1 bits, E=3.7e-99
• PF13193: AMP-binding_C
  • amino acids 470 to 545 (76 residues), 67.4 bits, E=1.7e-22

Best Hits

Swiss-Prot: 55% identical to LCFA_ECOL6: Long-chain-fatty-acid--CoA ligase (fadD) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a1231)

MetaCyc: 84% identical to long-chain-fatty-acid--CoA ligase (Pseudomonas aeruginosa PAO1)
Butyrate--CoA ligase. [EC: 6.2.1.2, 6.2.1.3]

Predicted SEED Role

"Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)" in subsystem Biotin biosynthesis or n-Phenylalkanoic acid degradation (EC 6.2.1.3)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• oleate β-oxidation (30/35 steps found)
• fatty acid salvage (6/6 steps found)
• 2-methyl-branched fatty acid β-oxidation (11/14 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• γ-linolenate biosynthesis II (animals) (2/2 steps found)
• valproate β-oxidation (7/9 steps found)
• octane oxidation (4/5 steps found)
• long-chain fatty acid activation (1/1 steps found)
• palmitate biosynthesis III (21/29 steps found)
• phytol degradation (3/4 steps found)
• stearate biosynthesis IV (4/6 steps found)
• linoleate biosynthesis II (animals) (1/2 steps found)
• alkane biosynthesis II (1/3 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• long chain fatty acid ester synthesis (engineered) (1/4 steps found)
• phosphatidylcholine acyl editing (1/4 steps found)
• wax esters biosynthesis II (1/4 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• 2-deoxy-D-ribose degradation II (3/8 steps found)
• arachidonate biosynthesis III (6-desaturase, mammals) (2/7 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• icosapentaenoate biosynthesis II (6-desaturase, mammals) (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• stearate biosynthesis I (animals) (1/6 steps found)
• capsaicin biosynthesis (1/7 steps found)
• icosapentaenoate biosynthesis III (8-desaturase, mammals) (1/7 steps found)
• 4-oxopentanoate degradation (2/9 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
• sporopollenin precursors biosynthesis (4/18 steps found)
• cutin biosynthesis (1/16 steps found)
• suberin monomers biosynthesis (2/20 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Fatty acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.3

Use Curated BLAST to search for 6.2.1.2 or 6.2.1.3

Sequence Analysis Tools

See A0A0N9WQG7 at UniProt or InterPro

Protein Sequence (566 amino acids)

>AO353_03310 long-chain fatty acid--CoA ligase (Pseudomonas fluorescens FW300-N2E3)
MIEDFWKDKYPAGIAAEINPDEYPNIQAVLKQSCQRFANKPAFSNLGKTLTYGELYELSG
AFAAYLQQHTDLQPGDRIAVQLPNLLQYPIAVFGAIRAGLIVVNTNPLYTAREMEHQFND
SGAKALVCLANMAHLAEAVVPKTGVKHVIVTEVADLLPPLKRLLINSVIKYVKKMVPAYH
LPKAVKFNDVLSKGHGQPVTEANPASSDVAVLQYTGGTTGVAKGAMLTHRNLVANMMQCR
ALMGSNLNEGCEILITPLPLYHIYAFTFHCMAMMLIGNHNILISNPRDLPAMVKELSKWK
FSGFVGLNTLFVALCNNEAFRKLDFSGLKITLSGGMALQLAAAERWKAVTGCAICEGYGM
TETSPVATVNPNQNIQIGTIGIPVPSTLCKVIDDAGVEQPLGEIGELCVKGPQVMKGYWQ
RQEATDEILDSEGWLKTGDIALIQPDGYMRIVDRKKDMILVSGFNVYPNELEDVLATLPG
VLQCAAIGVPDEKSGEAIKIFIVAKPGVTLTKEQVMTHMRANVTGYKVPRAIEFRDALPT
TNVGKILRRELRDEELKKLGLKKVSA