Protein Info for AO353_03060 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): Acetyl-coenzyme A synthetase (EC 6.2.1.1)
Rationale: Specifically important for utilizing Ethanol. Automated validation from mutant phenotype: the predicted function (ACETATE--COA-LIGASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: acetyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 20 to 643 (624 residues), 1013.3 bits, E=0
• PF16177: ACAS_N
  • amino acids 24 to 81 (58 residues), 82.4 bits, 2.4e-27
• PF00501: AMP-binding
  • amino acids 83 to 466 (384 residues), 269.9 bits, E=4.9e-84
• PF13193: AMP-binding_C
  • amino acids 531 to 609 (79 residues), 91.9 bits, E=5.9e-30

Best Hits

Swiss-Prot: 91% identical to ACSA1_PSEPK: Acetyl-coenzyme A synthetase 1 (acsA1) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 96% identity to pfl:PFL_4522)

MetaCyc: 68% identical to acetyl-CoA synthetase (AMP-forming) (Escherichia coli K-12 substr. MG1655)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• β-alanine biosynthesis II (5/6 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• chitin deacetylation (2/4 steps found)
• acrylate degradation II (1/3 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• cis-geranyl-CoA degradation (4/9 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Sequence Analysis Tools

See A0A0N9VPL2 at UniProt or InterPro

Protein Sequence (651 amino acids)

>AO353_03060 Acetyl-coenzyme A synthetase (EC 6.2.1.1) (Pseudomonas fluorescens FW300-N2E3)
MSAASLYPVRPEVAANTLTDEATYKAMYQQSVVNPDGFWREQAKRLDWIKPFTTVKQTSF
DDHHVDIKWFADGTLNVSYNCLDRHLAERGDQIAIIWEGDDPAESRNITYRELHEQVCKF
ANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKS
KVVITADEGLRAGKKISLKANVDDALTNPETSSIQKVIVCKRTGGNIKWNQHRDIWYEDL
MKVAGTVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEI
YWCTADVGWVTGHTYIVYGPLANGATTLLFEGVPNYPDITRVAKIIDKHKVNILYTAPTA
IRAMMAQGTAAVEGADGSSLRLLGSVGEPINPEAWEWYYKNVGQSRCPIVDTWWQTETGA
TLMSPLPGAHGLKPGSAARPFFGVVPALVDNLGNIIEGVAEGNLVILDSWPGQARTLYGD
HDRFVDTYFKTFRGMYFTGDGARRDADGYWWITGRVDDVLNVSGHRMGTAEIESAMVAHP
KVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPSEALRLELKNWVRKEIGPIASPDVIQWAP
GLPKTRSGKIMRRILRKIATGEYDGLGDISTLADPGVVQHLIDTHKTMNVA