Protein Info for AO353_02800 in Pseudomonas fluorescens FW300-N2E3

Annotation: C4-dicarboxylate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 44 to 66 (23 residues), see Phobius details amino acids 78 to 101 (24 residues), see Phobius details amino acids 138 to 169 (32 residues), see Phobius details amino acids 190 to 215 (26 residues), see Phobius details amino acids 221 to 242 (22 residues), see Phobius details amino acids 262 to 280 (19 residues), see Phobius details amino acids 300 to 319 (20 residues), see Phobius details amino acids 330 to 348 (19 residues), see Phobius details amino acids 353 to 376 (24 residues), see Phobius details amino acids 387 to 403 (17 residues), see Phobius details PF00375: SDF" amino acids 11 to 403 (393 residues), 389.4 bits, see alignment E=9.5e-121

Best Hits

Swiss-Prot: 92% identical to DCTA_PSECL: C4-dicarboxylate transport protein (dctA) from Pseudomonas chlororaphis

KEGG orthology group: K11103, aerobic C4-dicarboxylate transport protein (inferred from 92% identity to pfo:Pfl01_4236)

MetaCyc: 75% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

"C4-dicarboxylate transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VPF8 at UniProt or InterPro

Protein Sequence (444 amino acids)

>AO353_02800 C4-dicarboxylate transporter (Pseudomonas fluorescens FW300-N2E3)
MTTRQPLYKSLYFQVIIAIAIGIALGHYYPQTAVALKPLGDGFIKLIKMVIAPIIFCTVV
SGIAGMQNMKSVGKTGGYALLYFEIVSTIALLVGLVVVNIVQPGNGMHIDVSTLDASKVA
AYVAAGADQSVVGFLLNIIPATIVGAFANGDILQVLMFSVIFGFALHRLGAYGKPILDFI
DRFAHVMFNIINMIMKLAPIGALGAMAFTIGAYGVGSLVQLGQLMACFYITCVVFILVVL
GGIARAHGFSVLKVIRYIREELLIVLGTSSSESVLPRMLIKMERLGAKKSVVGLVIPTGY
SFNLDGTAIYLTMAAVFIAQATDTHMDITHQITLLVVLLLSSKGAAGVTGSGFIVLAATL
SAVGHLPVAGLALILGIDRFMSEARALTNLVGNAVATLVVAKWVKELDVDQMHEELASGG
RAITNTHGEDDAHVAESPAPTSVK