Protein Info for AO353_02710 in Pseudomonas fluorescens FW300-N2E3

Annotation: (p)ppGpp synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 PF13328: HD_4" amino acids 40 to 202 (163 residues), 153.5 bits, see alignment E=1e-48 TIGR00691: RelA/SpoT family protein" amino acids 69 to 742 (674 residues), 617 bits, see alignment E=2.1e-189 PF04607: RelA_SpoT" amino acids 262 to 370 (109 residues), 149 bits, see alignment E=1.5e-47 PF02824: TGS" amino acids 416 to 475 (60 residues), 81.5 bits, see alignment 9.3e-27 PF19296: RelA_AH_RIS" amino acids 494 to 638 (145 residues), 34.8 bits, see alignment E=4.7e-12 PF13291: ACT_4" amino acids 667 to 743 (77 residues), 65.2 bits, see alignment E=1.7e-21

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 96% identity to pba:PSEBR_a4258)

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WED7 at UniProt or InterPro

Protein Sequence (747 amino acids)

>AO353_02710 (p)ppGpp synthetase (Pseudomonas fluorescens FW300-N2E3)
MVQVRAHQPINTDGSINLEAWLDHAVSVDPALDREALKEACEFARQAEQQDKAAKNLWAE
GTSSFRTGLEIAEILADLKLDQDSLVAAILYRGVREGHIQLPAVSQRFGPVVAKLIDGVQ
RMAAISASLSPRQSLVLGSQGQVENLRKMLVAMVDDVRVALIKLAERTCAIRAVKTADDE
KRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFI
SDVMNQLQAELQATGVDADISGRAKHIYSIWRKMQRKGLEFSQIYDVRAVRVLVPEMRDC
YTALGIVHTLWRHIPKEFDDYIANPKENGYRSLHTAVIGPEGKVLEVQIRTHAMHEEAEL
GVCAHWRYKGTDVKSGSNHYEEKISWLRQVLEWHEELGDIGGLAEQLRVDIEPDRVYIFT
PDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGT
PSRDWLNPNLGYITTSRARAKIVHWFKLQARDQNVAAGKTLFERELNRLGLPQVDFEKLA
DKANMKTAEDMFAALGAGDLRLAQLVNLAQQLVEPERGNEQLELIPRKATGYKPGKRGDI
QIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQDCASVLQLAGREPERIIQVSW
GPVPVLTYPVDIIIRAYDRSGLLRDVSQVLLNERINVLAVNTRSNKEDNTALMSLTIEIP
GLDALGRLLGRISQLPNIIETRRNRTP