Protein Info for AO353_02625 in Pseudomonas fluorescens FW300-N2E3
Annotation: cobalamin biosynthesis protein CobD
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 76% identical to COBD_PSEAE: Cobalamin biosynthesis protein CobD (cobD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K02227, adenosylcobinamide-phosphate synthase CobD [EC: 6.3.1.10] (inferred from 92% identity to pfl:PFL_4428)Predicted SEED Role
"Adenosylcobinamide-phosphate synthase (EC 6.3.1.10)" (EC 6.3.1.10)
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
- adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (6/6 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide II (8/9 steps found)
- adenosylcobinamide-GDP salvage from cobinamide II (5/6 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.1.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9WDH2 at UniProt or InterPro
Protein Sequence (302 amino acids)
>AO353_02625 cobalamin biosynthesis protein CobD (Pseudomonas fluorescens FW300-N2E3) MSVALLSVAGVALDALLGEPKRWHPLVAFGRFADRIEQRFNSAGRGWRSHGVTAWVIAVL PLTLLITALSWLPYLGWIVEILALYCALGLRSLSEHVEPVAQALRSDDLDEARKRVGYLV SRQTSELDSTEVARAATESVLENGSDAVFAALFWFVVAGAPGVVLYRLSNTLDAMWGYRN ERFERFGWAAAKIDDVLNYIPARLVALTYALLGKTRLALKCWRTQGPTWDSPNAGPVMAA GAGALGVELGGAAIYHGELHQRPQLGEGVPADANSIDRGWQLVQRGVWLWLLILCVGAEI YA