Protein Info for AO353_02445 in Pseudomonas fluorescens FW300-N2E3

Annotation: diguanylate phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF00563: EAL" amino acids 11 to 239 (229 residues), 189 bits, see alignment E=1.3e-59 PF00571: CBS" amino acids 275 to 323 (49 residues), 24.9 bits, see alignment 3.2e-09 PF00990: GGDEF" amino acids 406 to 509 (104 residues), 48.4 bits, see alignment E=1.4e-16 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 416 to 497 (82 residues), 47.6 bits, see alignment E=7.5e-17

Best Hits

KEGG orthology group: None (inferred from 93% identity to pfo:Pfl01_1678)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VPT1 at UniProt or InterPro

Protein Sequence (591 amino acids)

>AO353_02445 diguanylate phosphodiesterase (Pseudomonas fluorescens FW300-N2E3)
MTTTEQLSALSSILAQSGLHSLFQPIVSLSERRILGYEALSRGPSNNPLHSPIALFAVAR
QVGRLSELEMACRHSACRRFNEQQLPGKLFLNVSPESLLEAGHQPGRTLQMLQDFGIPPS
QVVIELTEQTPTDDFQLLQNALHHYRAMGFSIALDDLGAGYSSLRLWSELRPDYVKIDRH
FIDGIHQDALKREFVGSILQIAKASRAQVIAEGIELPEELAVLTEMGVDLVQGYLLCRPQ
EHPPRDARTMMPKHDSSAVALNDEGSDLSALLNEHPAVHRDTPTADVLEAFRRQANLNSL
AVLDELGQPCGIVHRHSLSDALLKPFATDLFARKPISRLMSDDFLAVELSQSLQQVSRLI
TSRARQRIEEDFIITLNAGYLGLGRVIDVLKLITELKIQQARYANPLTLLPGNVPIQQCL
SRLLQQSRESVICYVDIDSFKPFNDIYGYGRGDEVLLCLAQCLNDRVDPSRDFVGHIGGD
DFLLVLGPEDWRKRLNQLLEDFHSQCRRFYRSEHLEAGCFIAPNRQGVRQEFALLSLSIG
VVHLHPEACGQLDASQLAEMASQAKHHAKNVQGGSIYVIDSLEVEPQLHSL