Protein Info for AO353_02245 in Pseudomonas fluorescens FW300-N2E3

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 381 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 44 to 59 (16 residues), see Phobius details amino acids 71 to 89 (19 residues), see Phobius details amino acids 94 to 114 (21 residues), see Phobius details amino acids 132 to 151 (20 residues), see Phobius details amino acids 158 to 177 (20 residues), see Phobius details amino acids 199 to 218 (20 residues), see Phobius details amino acids 235 to 255 (21 residues), see Phobius details amino acids 267 to 284 (18 residues), see Phobius details amino acids 288 to 314 (27 residues), see Phobius details amino acids 327 to 346 (20 residues), see Phobius details amino acids 352 to 373 (22 residues), see Phobius details PF12832: MFS_1_like" amino acids 6 to 359 (354 residues), 372.8 bits, see alignment E=3.1e-115 PF03825: Nuc_H_symport" amino acids 8 to 372 (365 residues), 52.9 bits, see alignment E=4.8e-18 PF07690: MFS_1" amino acids 12 to 245 (234 residues), 43.7 bits, see alignment E=2.8e-15 amino acids 208 to 375 (168 residues), 48.7 bits, see alignment E=8.2e-17

Best Hits

KEGG orthology group: K05820, MFS transporter, PPP family, 3-phenylpropionic acid transporter (inferred from 93% identity to pfo:Pfl01_1723)

Predicted SEED Role

"Nucleoside:H+ symporter:Major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WD99 at UniProt or InterPro

Protein Sequence (381 amino acids)

>AO353_02245 MFS transporter (Pseudomonas fluorescens FW300-N2E3)
VAALPYWRLSSFYLFYFALLGSTAPFLALYFDHLGFSSARIGELVAIPMLMRCVAPNIWG
WLGDYTGRRLAIVRFGAICTLLTFSLIFVSKSYAWLAMVMALHAFFWHAVLPQFEVITLA
HLQGQTSRYSQIRLWGSIGFIITVVALGRLFEWLSLDIYPVTLVVIMGGIIISSLWVPNA
QPAQGNRLAGEGFLKQLRSPGVLAFYGCVALMQMSHGPYYTFLTLHLEHLGYSRGVIGML
WALGVVAEVLMFLFMSKILGRFSVRRVLMASFLLAALRWLLLGSFAEFLWVLLFAQILHA
ATFGSFHAAAIHFVQRSFGARQQGQGQALYAALAGTGGALGALYSGYSWNALGATLTFSI
ASLAAFAAAVIIATRMQEDRP