Protein Info for AO353_02160 in Pseudomonas fluorescens FW300-N2E3

Annotation: polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 507 TIGR03708: polyphosphate:AMP phosphotransferase" amino acids 2 to 491 (490 residues), 733.5 bits, see alignment E=1.8e-224 PF03976: PPK2" amino acids 11 to 236 (226 residues), 174.3 bits, see alignment E=1.6e-55 amino acids 269 to 491 (223 residues), 311.7 bits, see alignment E=1.5e-97

Best Hits

Swiss-Prot: 80% identical to PK22_PSESM: Polyphosphate:AMP phosphotransferase (PSPTO_1640) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00947, [EC: 2.7.4.-] (inferred from 88% identity to pfo:Pfl01_4097)

Predicted SEED Role

"UDP-galactose-lipid carrier transferase (EC 2.-.-.-)" (EC 2.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-, 2.7.4.-

Use Curated BLAST to search for 2.-.-.- or 2.7.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W6P4 at UniProt or InterPro

Protein Sequence (507 amino acids)

>AO353_02160 polyphosphate kinase (Pseudomonas fluorescens FW300-N2E3)
MFESAEIGHVIDKETYDAEVPALREALLEAQFDLQQQARFPVIVLINGIEGAGKGETVKL
LNEWMDPRLIEVRTFDQQTDEELARPPAWRYWRMLPAKGRMGVFFGNWYSQMLQGRVHGL
FKDAVLDQAINQTERLEKMLCDEGAVIFKFWFHLSKQQMKARLKSLQDDPLHSWRISPLD
WQQSKTYDKFVQYGERVIRRTSRDYAPWHVIEGVDAHYRSLTVGKILLDGLQNALKRPKI
NPDKVSAAPLSASVDHLSLLGSLDMSQRLEKDDYEEQLITEQARLSGLMRDKRMRRHALI
AVFEGNDAAGKGGAIRRVAAALDPRQYSIVPIAAPTEDERAQPYLWRFWRQIPARGKFTV
FDRSWYGRVLVERVEGFCSQADWLRAYGEINDFEEQISASGVIVVKFWLAIDKQTQLERF
QEREAIPFKRFKITEDDWRNREKWDDYRTAVGDMVDRTSTEVSPWTLVEANDKRWARVKV
LRTLNRALEEAFEKSDKDQKKLKKDKK