Protein Info for AO353_02070 in Pseudomonas fluorescens FW300-N2E3

Annotation: prephenate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01807: chorismate mutase
  • amino acids 6 to 84 (79 residues), 104.6 bits, E=1.3e-34
• PF01817: CM_2
  • amino acids 10 to 92 (83 residues), 70.4 bits, E=2e-23
• PF00800: PDT
  • amino acids 96 to 272 (177 residues), 216.3 bits, E=4.5e-68
• PF01842: ACT
  • amino acids 282 to 346 (65 residues), 48.4 bits, E=9.7e-17

Best Hits

Swiss-Prot: 89% identical to CMPDT_PSEAE: Bifunctional chorismate mutase/prephenate dehydratase (pheA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K14170, chorismate mutase / prephenate dehydratase [EC: 4.2.1.51 5.4.99.5] (inferred from 98% identity to pfs:PFLU1645)

Predicted SEED Role

"Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51)" in subsystem Chorismate Synthesis or Phenylalanine and Tyrosine Branches from Chorismate (EC 4.2.1.51, EC 5.4.99.5)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• L-phenylalanine biosynthesis I (3/3 steps found)
• L-tyrosine biosynthesis I (3/3 steps found)
• superpathway of chorismate metabolism (43/59 steps found)
• L-phenylalanine biosynthesis III (cytosolic, plants) (2/2 steps found)
• L-phenylalanine biosynthesis II (3/4 steps found)
• L-tyrosine biosynthesis III (3/4 steps found)
• superpathway of L-phenylalanine and L-tyrosine biosynthesis (3/5 steps found)
• L-tyrosine biosynthesis II (2/4 steps found)
• bacilysin biosynthesis (1/10 steps found)
• salinosporamide A biosynthesis (4/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.51, 5.4.99.5

Use Curated BLAST to search for 4.2.1.51 or 5.4.99.5

Sequence Analysis Tools

See A0A0N9WC79 at UniProt or InterPro

Protein Sequence (364 amino acids)

>AO353_02070 prephenate dehydratase (Pseudomonas fluorescens FW300-N2E3)
MSEQELKALRLRIDALDEKILELISERARCAQEVARVKMATLAEGEVPVFYRPEREAQVL
KRVMERNQGPLGNEEMARLFREIMSSCLALEQPLKVAYLGPEGTFTQAAAMKHFGHAVIS
KPMAAIDEVFREVAAGAVNFGVVPVENSTEGAVNHTLDSFLEHDMVICGEVELRIHHHLL
VGENTKTDSISRIYSHAQSLAQCRKWLDAHYPNVERVAVSSNAEAAKRVKGEWNSAAIAG
DMAAGLYGLTRLAEKIEDRPDNSTRFLMIGSQEVPPTGDDKTSIIVSMSNKPGALHELLV
PFHDNGIDLTRIETRPSRSGKWTYVFFIDFVGHHRDPLIKAVLEKISQEAVALKVLGSYP
KAVL