Protein Info for AO353_02065 in Pseudomonas fluorescens FW300-N2E3

Annotation: aspartate aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 93% identical to HIS82_PSEPF: Histidinol-phosphate aminotransferase 2 (hisC2) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00817, histidinol-phosphate aminotransferase [EC: 2.6.1.9] (inferred from 93% identity to pfo:Pfl01_4075)

Predicted SEED Role

"Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57)" in subsystem Phenylalanine and Tyrosine Branches from Chorismate (EC 2.6.1.57)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
superpathway of aromatic amino acid biosynthesis (18/18 steps found)
L-histidine biosynthesis (10/10 steps found)
superpathway of L-phenylalanine biosynthesis (10/10 steps found)
superpathway of L-tyrosine biosynthesis (10/10 steps found)
L-tyrosine degradation I (5/5 steps found)
L-phenylalanine biosynthesis I (3/3 steps found)
L-tyrosine biosynthesis I (3/3 steps found)
superpathway of chorismate metabolism (43/59 steps found)
L-phenylalanine biosynthesis III (cytosolic, plants) (2/2 steps found)
3-(4-hydroxyphenyl)pyruvate biosynthesis (1/1 steps found)
L-phenylalanine biosynthesis II (3/4 steps found)
L-tyrosine biosynthesis III (3/4 steps found)
L-phenylalanine degradation II (anaerobic) (2/3 steps found)
L-tyrosine degradation II (1/2 steps found)
atromentin biosynthesis (1/2 steps found)
superpathway of L-phenylalanine and L-tyrosine biosynthesis (3/5 steps found)
L-phenylalanine degradation III (2/4 steps found)
L-tyrosine biosynthesis II (2/4 steps found)
L-tyrosine degradation III (2/4 steps found)
L-tyrosine degradation IV (to 4-methylphenol) (1/3 steps found)
superpathway of plastoquinol biosynthesis (2/5 steps found)
4-hydroxybenzoate biosynthesis I (eukaryotes) (1/5 steps found)
L-phenylalanine degradation VI (reductive Stickland reaction) (1/5 steps found)
L-tyrosine degradation V (reductive Stickland reaction) (1/5 steps found)
L-phenylalanine degradation IV (mammalian, via side chain) (3/9 steps found)
rosmarinic acid biosynthesis I (1/10 steps found)
(S)-reticuline biosynthesis I (1/11 steps found)
tropane alkaloids biosynthesis (1/11 steps found)
superpathway of hyoscyamine (atropine) and scopolamine biosynthesis (3/16 steps found)
superpathway of rosmarinic acid biosynthesis (1/14 steps found)
anaerobic aromatic compound degradation (Thauera aromatica) (2/27 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.57, 2.6.1.9

Use Curated BLAST to search for 2.6.1.57 or 2.6.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WD67 at UniProt or InterPro

Protein Sequence (370 amino acids)

>AO353_02065 aspartate aminotransferase (Pseudomonas fluorescens FW300-N2E3)
MSGDFLALAQPGVQQLSPYVPGKPVDELARELDIDPAKIVKLASNENPLGASPKALAAIR
EELAELTRYPDGNGFALKTLLAEQCRVELNQVTLGNGSNDILELVARAYLAPGLNAVFSE
HAFAVYPIVTQAVGAEARVIPAKDWGHDLSAMLEAIDSNTRVVFIANPNNPTGTWFDAEA
LDEFLQDVPERVLVVLDEAYIEYAEGSDLPDGLDFLAAYPNLLVSRTFSKAYGLAALRVG
YGLSTAVVADVLNRVRQPFNVNSLALAAACAALQDDEYLAESRRLNESGMQQLEAGFREL
GLSWIPSKGNFICVDLGRVAAPVFQGLLHEGVIVRPVANYGMPNHLRITIGLPAENSRFL
EALAKVLARG